Inhibition of S6K1 enhances glucose deprivation-induced cell death via downregulation of anti-apoptotic proteins in MCF-7 breast cancer cells

• Published in: Biochemical and biophysical research communications 2013-03, Vol.432 (1), p.123-128
• Main Authors: Choi, Ha-Na, Jin, Hyeon-Ok, Kim, Jae-Hee, Hong, Sung-Eun, Kim, Hyun-Ah, Kim, Eun-Kyu, Lee, Jin Kyung, Park, In-Chul, Noh, Woo Chul
• Format: Article
• Language: English
• Subjects: Apoptosis - drug effects; Apoptosis Regulatory Proteins - metabolism; Breast cancer; Breast Neoplasms - enzymology; Breast Neoplasms - metabolism; Down-Regulation; Female; Gene Knockdown Techniques; Glucose - deficiency; Glucose deprivation; Humans; Imidazoles - pharmacology; Inhibitor of Apoptosis Proteins - metabolism; MCF-7 Cells; Mcl-1; Myeloid Cell Leukemia Sequence 1 Protein; Niacinamide - analogs & derivatives; Niacinamide - pharmacology; Phenylurea Compounds - pharmacology; Piperazines - pharmacology; Protein Kinase Inhibitors - pharmacology; Proto-Oncogene Proteins c-bcl-2 - metabolism; Ribosomal Protein S6 Kinases, 70-kDa - antagonists & inhibitors; Ribosomal Protein S6 Kinases, 70-kDa - genetics; S6K1; Survivin; TOR Serine-Threonine Kinases - antagonists & inhibitors
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2013.01.074

► Inhibition of S6K1 sensitizes glucose deprivation induced cell death. ► The sensitization is through the downregulation of Mcl-1 and survivin. ► Inhibition of S6K1 may be a strategy for sensitizing cells to glucose deprivation. Nutrient-limiting conditions are frequently encountered by tumor cells in poorly vascularized microenvironments. These stress conditions may facilitate the selection of tumor cells with an inherent ability to decrease apoptotic potential. Therefore, selective targeting of tumor cells under glucose deprivation conditions may provide an effective alternative strategy for cancer therapy. In the present study, we investigated the effects of S6 kinase 1 (S6K1) inhibition on glucose deprivation-induced cell death and the underlying mechanisms in MCF-7 breast cancer cells. PF4708671, a selective inhibitor of S6K1, and knockdown of S6K1 with specific siRNA enhanced cell death induced under glucose deprivation conditions. Moreover, inhibition of S6K1 led to apoptosis in glucose-starved MCF-7 cells via downregulation of the anti-apoptotic proteins, Mcl-1 and survivin. Further experiments revealed that sorafenib, shown to be involved in Mcl-1 and survivin downregulation via mTOR/S6K1 inhibition significantly promotes cell death under glucose deprivation conditions. These findings collectively suggest that S6K1 plays an important role in tumor cell survival under stress conditions, and thus inhibition of S6K1 may be an effective strategy for sensitizing cells to glucose deprivation.