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Periodontal tissue engineering with stem cells from the periodontal ligament of human retained deciduous teeth
Background and Objective: Periodontal ligament stem cells from human permanent teeth (PePDLSCs) have been investigated extensively in periodontal tissue engineering and regeneration. However, little knowledge is available on the periodontal ligament stem cells from human retained deciduous teeth (D...
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| Published in: | Journal of periodontal research 2013-02, Vol.48 (1), p.105-116 |
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| cites | cdi_FETCH-LOGICAL-c4709-5ff52d84acd4ad2aa017c8eed4c685601098874bd3e5af6bee6575ba105b43503 |
| container_end_page | 116 |
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| container_start_page | 105 |
| container_title | Journal of periodontal research |
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| creator | Ji, K. Liu, Y. Lu, W. Yang, F. Yu, J. Wang, X. Ma, Q. Yang, Z. Wen, L. Xuan, K. |
| description | Background and Objective: Periodontal ligament stem cells from human permanent teeth (PePDLSCs) have been investigated extensively in periodontal tissue engineering and regeneration. However, little knowledge is available on the periodontal ligament stem cells from human retained deciduous teeth (DePDLSCs). This study evaluated the potential of DePDLSCs in periodontal tissue regeneration.
Material and Methods: DePDLSCs were isolated and purified by limited dilution. The characteristics of DePDLSCs were evaluated and compared with PePDLSCs both in vitro and in vivo.
Results: DePDLSCs presented a higher proliferation rate and colony‐forming capacity than PePDLSCs in vitro. During the osteogenic induction, alkaline phosphatase (ALP) activity, mineralized matrix formation and expression of mineralization‐related genes, including runt‐related transcription factor 2 (RUNX2), ALP, collagen type I (COLI) and osteocalcin (OCN) were significantly enhanced in DePDLSCs compared with PePDLSCs. Furthermore, DePDLSC cell sheets showed a stronger synthesis of collagen type I in the extracellular matrix than did PePDLSC cell sheets. After in vivo transplantation, DePDLSC cell sheets recombined with human dentin blocks were able to generate new cementum/periodontal ligament‐like tissues.
Conclusion: Our findings suggest that DePDLSCs can be used as a promising candidate for periodontal tissue engineering. |
| doi_str_mv | 10.1111/j.1600-0765.2012.01509.x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1315605566</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1315605566</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4709-5ff52d84acd4ad2aa017c8eed4c685601098874bd3e5af6bee6575ba105b43503</originalsourceid><addsrcrecordid>eNqNkUtv1DAUhS0EokPhLyBvkNgktRO_smCBqrZQSoECQmJjOfHNjIc8BttRp_8epzMM7MAbv75zfH0PQpiSnKZxss6pICQjUvC8ILTICeWkyrcP0OJw8RAtCCmKrGSKHaEnIaxJ2gtZPUZHRaEULRlboOEjeDfacYimw9GFMAGGYekGSOfDEt-6uMIhQo8b6LqAWz_2OK4Ab_7SdW5pehgiHlu8mnozYA_RJA-LLTTOTuMUcASIq6foUWu6AM_28zH6en725fRNdvXh4u3p66usYZJUGW9bXljFTGOZsYUxhMpGAVjWCMUFoaRSSrLalsBNK2oAwSWvDSW8ZiUn5TF6ufPd-PHnBCHq3oX5B2aAVIymJU02nAvxb7RQomKcyhlVO7TxYwgeWr3xrjf-TlOi52D0Ws_913P_9RyMvg9Gb5P0-f6Vqe7BHoS_k0jAiz1gQmO61puhceEPJ6Qi8p57teNuXQd3_12Avrw5m1dJn-30LqW6PeiN_6GFLCXX364vNPl0_f39zbvPuih_AaXGuZc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1286945176</pqid></control><display><type>article</type><title>Periodontal tissue engineering with stem cells from the periodontal ligament of human retained deciduous teeth</title><source>Wiley:Jisc Collections:Wiley Read and Publish Open Access 2024-2025 (reading list)</source><creator>Ji, K. ; Liu, Y. ; Lu, W. ; Yang, F. ; Yu, J. ; Wang, X. ; Ma, Q. ; Yang, Z. ; Wen, L. ; Xuan, K.</creator><creatorcontrib>Ji, K. ; Liu, Y. ; Lu, W. ; Yang, F. ; Yu, J. ; Wang, X. ; Ma, Q. ; Yang, Z. ; Wen, L. ; Xuan, K.</creatorcontrib><description>Background and Objective: Periodontal ligament stem cells from human permanent teeth (PePDLSCs) have been investigated extensively in periodontal tissue engineering and regeneration. However, little knowledge is available on the periodontal ligament stem cells from human retained deciduous teeth (DePDLSCs). This study evaluated the potential of DePDLSCs in periodontal tissue regeneration.
Material and Methods: DePDLSCs were isolated and purified by limited dilution. The characteristics of DePDLSCs were evaluated and compared with PePDLSCs both in vitro and in vivo.
Results: DePDLSCs presented a higher proliferation rate and colony‐forming capacity than PePDLSCs in vitro. During the osteogenic induction, alkaline phosphatase (ALP) activity, mineralized matrix formation and expression of mineralization‐related genes, including runt‐related transcription factor 2 (RUNX2), ALP, collagen type I (COLI) and osteocalcin (OCN) were significantly enhanced in DePDLSCs compared with PePDLSCs. Furthermore, DePDLSC cell sheets showed a stronger synthesis of collagen type I in the extracellular matrix than did PePDLSC cell sheets. After in vivo transplantation, DePDLSC cell sheets recombined with human dentin blocks were able to generate new cementum/periodontal ligament‐like tissues.
Conclusion: Our findings suggest that DePDLSCs can be used as a promising candidate for periodontal tissue engineering.</description><identifier>ISSN: 0022-3484</identifier><identifier>EISSN: 1600-0765</identifier><identifier>DOI: 10.1111/j.1600-0765.2012.01509.x</identifier><identifier>PMID: 22881344</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Alkaline Phosphatase - analysis ; Bicuspid ; Biological and medical sciences ; Calcification, Physiologic - physiology ; Cell Culture Techniques ; Cell Proliferation ; Cell Separation ; cell sheet ; Cementogenesis - physiology ; Child ; Collagen Type I - analysis ; Core Binding Factor Alpha 1 Subunit - analysis ; Culture Media ; Dentin - physiology ; Dentistry ; Extracellular Matrix - chemistry ; Facial bones, jaws, teeth, parodontium: diseases, semeiology ; Flow Cytometry ; Humans ; Incisor ; Medical sciences ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells - classification ; Multipotent Stem Cells - physiology ; Non tumoral diseases ; Osteocalcin - analysis ; Osteogenesis - physiology ; Otorhinolaryngology. Stomatology ; periodontal ligament ; Periodontal Ligament - cytology ; periodontal regeneration ; Periodontium - physiology ; Regeneration - physiology ; retained deciduous teeth ; stem cell ; Tissue Engineering - methods ; Tooth, Deciduous ; Young Adult</subject><ispartof>Journal of periodontal research, 2013-02, Vol.48 (1), p.105-116</ispartof><rights>2012 John Wiley & Sons A/S</rights><rights>2014 INIST-CNRS</rights><rights>2012 John Wiley & Sons A/S.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4709-5ff52d84acd4ad2aa017c8eed4c685601098874bd3e5af6bee6575ba105b43503</citedby><cites>FETCH-LOGICAL-c4709-5ff52d84acd4ad2aa017c8eed4c685601098874bd3e5af6bee6575ba105b43503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26780744$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22881344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ji, K.</creatorcontrib><creatorcontrib>Liu, Y.</creatorcontrib><creatorcontrib>Lu, W.</creatorcontrib><creatorcontrib>Yang, F.</creatorcontrib><creatorcontrib>Yu, J.</creatorcontrib><creatorcontrib>Wang, X.</creatorcontrib><creatorcontrib>Ma, Q.</creatorcontrib><creatorcontrib>Yang, Z.</creatorcontrib><creatorcontrib>Wen, L.</creatorcontrib><creatorcontrib>Xuan, K.</creatorcontrib><title>Periodontal tissue engineering with stem cells from the periodontal ligament of human retained deciduous teeth</title><title>Journal of periodontal research</title><addtitle>J Periodontal Res</addtitle><description>Background and Objective: Periodontal ligament stem cells from human permanent teeth (PePDLSCs) have been investigated extensively in periodontal tissue engineering and regeneration. However, little knowledge is available on the periodontal ligament stem cells from human retained deciduous teeth (DePDLSCs). This study evaluated the potential of DePDLSCs in periodontal tissue regeneration.
Material and Methods: DePDLSCs were isolated and purified by limited dilution. The characteristics of DePDLSCs were evaluated and compared with PePDLSCs both in vitro and in vivo.
Results: DePDLSCs presented a higher proliferation rate and colony‐forming capacity than PePDLSCs in vitro. During the osteogenic induction, alkaline phosphatase (ALP) activity, mineralized matrix formation and expression of mineralization‐related genes, including runt‐related transcription factor 2 (RUNX2), ALP, collagen type I (COLI) and osteocalcin (OCN) were significantly enhanced in DePDLSCs compared with PePDLSCs. Furthermore, DePDLSC cell sheets showed a stronger synthesis of collagen type I in the extracellular matrix than did PePDLSC cell sheets. After in vivo transplantation, DePDLSC cell sheets recombined with human dentin blocks were able to generate new cementum/periodontal ligament‐like tissues.
Conclusion: Our findings suggest that DePDLSCs can be used as a promising candidate for periodontal tissue engineering.</description><subject>Adolescent</subject><subject>Alkaline Phosphatase - analysis</subject><subject>Bicuspid</subject><subject>Biological and medical sciences</subject><subject>Calcification, Physiologic - physiology</subject><subject>Cell Culture Techniques</subject><subject>Cell Proliferation</subject><subject>Cell Separation</subject><subject>cell sheet</subject><subject>Cementogenesis - physiology</subject><subject>Child</subject><subject>Collagen Type I - analysis</subject><subject>Core Binding Factor Alpha 1 Subunit - analysis</subject><subject>Culture Media</subject><subject>Dentin - physiology</subject><subject>Dentistry</subject><subject>Extracellular Matrix - chemistry</subject><subject>Facial bones, jaws, teeth, parodontium: diseases, semeiology</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Incisor</subject><subject>Medical sciences</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal Stromal Cells - classification</subject><subject>Multipotent Stem Cells - physiology</subject><subject>Non tumoral diseases</subject><subject>Osteocalcin - analysis</subject><subject>Osteogenesis - physiology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>periodontal ligament</subject><subject>Periodontal Ligament - cytology</subject><subject>periodontal regeneration</subject><subject>Periodontium - physiology</subject><subject>Regeneration - physiology</subject><subject>retained deciduous teeth</subject><subject>stem cell</subject><subject>Tissue Engineering - methods</subject><subject>Tooth, Deciduous</subject><subject>Young Adult</subject><issn>0022-3484</issn><issn>1600-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNkUtv1DAUhS0EokPhLyBvkNgktRO_smCBqrZQSoECQmJjOfHNjIc8BttRp_8epzMM7MAbv75zfH0PQpiSnKZxss6pICQjUvC8ILTICeWkyrcP0OJw8RAtCCmKrGSKHaEnIaxJ2gtZPUZHRaEULRlboOEjeDfacYimw9GFMAGGYekGSOfDEt-6uMIhQo8b6LqAWz_2OK4Ab_7SdW5pehgiHlu8mnozYA_RJA-LLTTOTuMUcASIq6foUWu6AM_28zH6en725fRNdvXh4u3p66usYZJUGW9bXljFTGOZsYUxhMpGAVjWCMUFoaRSSrLalsBNK2oAwSWvDSW8ZiUn5TF6ufPd-PHnBCHq3oX5B2aAVIymJU02nAvxb7RQomKcyhlVO7TxYwgeWr3xrjf-TlOi52D0Ws_913P_9RyMvg9Gb5P0-f6Vqe7BHoS_k0jAiz1gQmO61puhceEPJ6Qi8p57teNuXQd3_12Avrw5m1dJn-30LqW6PeiN_6GFLCXX364vNPl0_f39zbvPuih_AaXGuZc</recordid><startdate>201302</startdate><enddate>201302</enddate><creator>Ji, K.</creator><creator>Liu, Y.</creator><creator>Lu, W.</creator><creator>Yang, F.</creator><creator>Yu, J.</creator><creator>Wang, X.</creator><creator>Ma, Q.</creator><creator>Yang, Z.</creator><creator>Wen, L.</creator><creator>Xuan, K.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201302</creationdate><title>Periodontal tissue engineering with stem cells from the periodontal ligament of human retained deciduous teeth</title><author>Ji, K. ; Liu, Y. ; Lu, W. ; Yang, F. ; Yu, J. ; Wang, X. ; Ma, Q. ; Yang, Z. ; Wen, L. ; Xuan, K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4709-5ff52d84acd4ad2aa017c8eed4c685601098874bd3e5af6bee6575ba105b43503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Alkaline Phosphatase - analysis</topic><topic>Bicuspid</topic><topic>Biological and medical sciences</topic><topic>Calcification, Physiologic - physiology</topic><topic>Cell Culture Techniques</topic><topic>Cell Proliferation</topic><topic>Cell Separation</topic><topic>cell sheet</topic><topic>Cementogenesis - physiology</topic><topic>Child</topic><topic>Collagen Type I - analysis</topic><topic>Core Binding Factor Alpha 1 Subunit - analysis</topic><topic>Culture Media</topic><topic>Dentin - physiology</topic><topic>Dentistry</topic><topic>Extracellular Matrix - chemistry</topic><topic>Facial bones, jaws, teeth, parodontium: diseases, semeiology</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Incisor</topic><topic>Medical sciences</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Mesenchymal Stromal Cells - classification</topic><topic>Multipotent Stem Cells - physiology</topic><topic>Non tumoral diseases</topic><topic>Osteocalcin - analysis</topic><topic>Osteogenesis - physiology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>periodontal ligament</topic><topic>Periodontal Ligament - cytology</topic><topic>periodontal regeneration</topic><topic>Periodontium - physiology</topic><topic>Regeneration - physiology</topic><topic>retained deciduous teeth</topic><topic>stem cell</topic><topic>Tissue Engineering - methods</topic><topic>Tooth, Deciduous</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ji, K.</creatorcontrib><creatorcontrib>Liu, Y.</creatorcontrib><creatorcontrib>Lu, W.</creatorcontrib><creatorcontrib>Yang, F.</creatorcontrib><creatorcontrib>Yu, J.</creatorcontrib><creatorcontrib>Wang, X.</creatorcontrib><creatorcontrib>Ma, Q.</creatorcontrib><creatorcontrib>Yang, Z.</creatorcontrib><creatorcontrib>Wen, L.</creatorcontrib><creatorcontrib>Xuan, K.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of periodontal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ji, K.</au><au>Liu, Y.</au><au>Lu, W.</au><au>Yang, F.</au><au>Yu, J.</au><au>Wang, X.</au><au>Ma, Q.</au><au>Yang, Z.</au><au>Wen, L.</au><au>Xuan, K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Periodontal tissue engineering with stem cells from the periodontal ligament of human retained deciduous teeth</atitle><jtitle>Journal of periodontal research</jtitle><addtitle>J Periodontal Res</addtitle><date>2013-02</date><risdate>2013</risdate><volume>48</volume><issue>1</issue><spage>105</spage><epage>116</epage><pages>105-116</pages><issn>0022-3484</issn><eissn>1600-0765</eissn><abstract>Background and Objective: Periodontal ligament stem cells from human permanent teeth (PePDLSCs) have been investigated extensively in periodontal tissue engineering and regeneration. However, little knowledge is available on the periodontal ligament stem cells from human retained deciduous teeth (DePDLSCs). This study evaluated the potential of DePDLSCs in periodontal tissue regeneration.
Material and Methods: DePDLSCs were isolated and purified by limited dilution. The characteristics of DePDLSCs were evaluated and compared with PePDLSCs both in vitro and in vivo.
Results: DePDLSCs presented a higher proliferation rate and colony‐forming capacity than PePDLSCs in vitro. During the osteogenic induction, alkaline phosphatase (ALP) activity, mineralized matrix formation and expression of mineralization‐related genes, including runt‐related transcription factor 2 (RUNX2), ALP, collagen type I (COLI) and osteocalcin (OCN) were significantly enhanced in DePDLSCs compared with PePDLSCs. Furthermore, DePDLSC cell sheets showed a stronger synthesis of collagen type I in the extracellular matrix than did PePDLSC cell sheets. After in vivo transplantation, DePDLSC cell sheets recombined with human dentin blocks were able to generate new cementum/periodontal ligament‐like tissues.
Conclusion: Our findings suggest that DePDLSCs can be used as a promising candidate for periodontal tissue engineering.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22881344</pmid><doi>10.1111/j.1600-0765.2012.01509.x</doi><tpages>12</tpages></addata></record> |
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| subjects | Adolescent Alkaline Phosphatase - analysis Bicuspid Biological and medical sciences Calcification, Physiologic - physiology Cell Culture Techniques Cell Proliferation Cell Separation cell sheet Cementogenesis - physiology Child Collagen Type I - analysis Core Binding Factor Alpha 1 Subunit - analysis Culture Media Dentin - physiology Dentistry Extracellular Matrix - chemistry Facial bones, jaws, teeth, parodontium: diseases, semeiology Flow Cytometry Humans Incisor Medical sciences Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells - classification Multipotent Stem Cells - physiology Non tumoral diseases Osteocalcin - analysis Osteogenesis - physiology Otorhinolaryngology. Stomatology periodontal ligament Periodontal Ligament - cytology periodontal regeneration Periodontium - physiology Regeneration - physiology retained deciduous teeth stem cell Tissue Engineering - methods Tooth, Deciduous Young Adult |
| title | Periodontal tissue engineering with stem cells from the periodontal ligament of human retained deciduous teeth |
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