Phylogenetic analysis of small ruminant lentiviruses detected in Slovenia

Small ruminant lentiviruses (SRLV), which belong to the Retroviridae family, infect goats and sheep worldwide. The aim of this study was to characterize the SRLV strains circulating in Slovenia, by phylogenetic analysis of two genomic regions, 1.8kb gag-pol fragment and 1.2kb pol fragment. The resul...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary microbiology 2013-02, Vol.162 (1), p.201-206
Main Authors: Kuhar, Urška, Barlič-Maganja, Darja, Grom, Jože
Format: Article
Language:English
Subjects:
1.2 kb pol fragment
1.8 kb gag-pol fragment
Animals
CAEV
Gene Products, pol - genetics
Genotype
Goat Diseases - epidemiology
Goat Diseases - genetics
Goat Diseases - virology
Goats
Lentivirus
Lentivirus - classification
Lentivirus - enzymology
Lentivirus - genetics
Molecular Epidemiology
MVV
Phylogenetic analysis
Phylogeny
Retroviridae
Ruminantia
Sheep
Sheep Diseases - epidemiology
Sheep Diseases - genetics
Sheep Diseases - virology
Sheep, Domestic
Slovenia - epidemiology
Small ruminant lentiviruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Small ruminant lentiviruses (SRLV), which belong to the Retroviridae family, infect goats and sheep worldwide. The aim of this study was to characterize the SRLV strains circulating in Slovenia, by phylogenetic analysis of two genomic regions, 1.8kb gag-pol fragment and 1.2kb pol fragment. The results of our study revealed that Slovenian SRLV strains are highly heterogeneous, with ovine strains belonging to genotype A and caprine strains to genotypes A and B. The closest relatives of sheep virus sequences from two flocks that clustered together (SLO 35, 36) were found to be in subtype A5. A cluster composed of four sheep virus sequences (SLO 31) was clearly divergent from all other subtypes in group A and could not be assigned to any of them. The virus sequences from one goat flock belonged solely to subtype B1, whereas virus sequences from more than one genotype were found to circulate within the other two goat flocks, belonging to subtype B1 (SLO 1 and SLO 37) and to genotype A (SLO 2 and 78–88g). Two goat virus sequences (SLO 2) were found to belong to genotype A and could not be assigned to existing subtypes. One goat virus sequence (37–88g) from flock 37 was clearly different from other sequences of this flock and was more closely related to genotype A sequences. We propose two new subtypes within genotype A, subtype A14 (SLO 2) and A15 (SLO 31).
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2012.08.024