Salinicola zeshunii sp. nov., a Moderately Halophilic Bacterium Isolated from Soil of a Chicken Farm

• Published in: Current microbiology 2013-02, Vol.66 (2), p.192-196
• Main Authors: Cao, Li, Yan, Qiuxiang, Ni, Haiyan, Hu, Gang, Hong, Qing, Li, Shunpeng
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Biotechnology; Chickens; China; Cluster Analysis; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; farms; fatty acids; Fatty Acids - analysis; Halomonadaceae - classification; Halomonadaceae - genetics; Halomonadaceae - isolation & purification; Halomonadaceae - physiology; Life Sciences; Microbiology; Microscopy, Electron, Transmission; Molecular Sequence Data; Nucleic Acid Hybridization; nucleotide sequences; Phylogenetics; Phylogeny; Poultry farming; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; soil; Soil Microbiology; Soil microorganisms; Taxonomy
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-012-0259-4

The taxonomic status of a moderately halophilic bacterium, strain N4ᵀ, isolated from soil of a chicken farm in China was determined. It was Gram-negative, non-spore-forming, motile, and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequence indicated that this strain belonged to the genus Salinicola, as it showed the highest sequence similarities to Salinicola salaries M27ᵀ (98.3 %), Salinicola socius SMB35ᵀ (98.1 %), and Salinicola halophilus CG4.1ᵀ (98.1 %). The major cellular fatty acids were C₁₆:₀ (25.6 %), C₁₈:₁ω7c (35.0 %), and C₁₉:₀ cyclo ω8c (11.9 %), which are properties shared by members of the genus Salinicola. The DNA G+C content of strain N4ᵀ was 69.1 mol %. The level of DNA–DNA relatedness between strain N4ᵀ and the other three type strains of the genus of Salinicola salaries M27ᵀ, Salinicola socius SMB35ᵀ, and Salinicola halophilus CG4.1ᵀ were 34.3, 28.7, and 26.9 %, respectively. Based on the results of phenotypic, chemotaxonomic, DNA–DNA relatedness, and phylogenetic analysis, strain N4ᵀ should be classified as a novel species of the genus Salinicola, for which the name Salinicola zeshunii sp. nov. is proposed, with strain N4ᵀ (=KACC 16602ᵀ = CCTCC AB 2012912ᵀ) as the type strain.