Aeromonas cavernicola sp. nov., isolated from fresh water of a brook in a cavern

Aeromonas P2973 was isolated from the water of a brook in a cavern in the Czech Republic. This isolate could not be biochemically identified at the species level, considering all updated species descriptions. Subsequent extensive phenotypic characterisation, DNA–DNA hybridisation, 16S rRNA gene sequ...

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Bibliographic Details
Published in:Current microbiology 2013-02, Vol.66 (2), p.197-204
Main Authors: Martínez-Murcia, Antonio, Beaz-Hidalgo, Roxana, Svec, Pavel, Saavedra, Ma José, Figueras, Ma José, Sedlacek, Ivo
Format: Article
Language:English
Subjects:
Aeromonas
Aeromonas - classification
Aeromonas - genetics
Aeromonas - isolation & purification
Aeromonas - physiology
Bacterial Proteins - genetics
Bacterial Typing Techniques
Bacteriology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Caverns
Caves
Cluster Analysis
Czech Republic
Deoxyribonucleic acid
DNA
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Fresh Water - microbiology
freshwater
genes
Life Sciences
Microbiology
Microscopy, Electron, Transmission
Molecular Sequence Data
Molecular Typing
Nucleic Acid Hybridization
Phylogenetics
Phylogeny
ribosomal RNA
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Taxonomy
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Summary:Aeromonas P2973 was isolated from the water of a brook in a cavern in the Czech Republic. This isolate could not be biochemically identified at the species level, considering all updated species descriptions. Subsequent extensive phenotypic characterisation, DNA–DNA hybridisation, 16S rRNA gene sequencing and a Multi-Locus Phylogenetic Analysis (MLPA) of the concatenated sequence of 7 housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX and atpD; 4705 bp) was employed in an attempt to ascertain the taxonomy of this isolate. Based on this polyphasic approach, we describe a novel species of the genus Aeromonas, for which the name Aeromonas cavernicola sp. nov. is proposed, with strain CCM7641ᵀ (DSM24474ᵀ, CECT7862ᵀ) as the type strain.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-012-0253-x