Accelerated Determination of Selenomethionine in Selenized Yeast: Validation of Analytical Method

The purpose of this study was to reduce the extraction time, to hours instead of days, for quantification of the selenomethionine (SeMet) content of selenized yeast. An accelerated method using microwave-assisted enzymatic extraction and ultrasonication was optimized and applied to certified referen...

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Bibliographic Details
Published in:Biological trace element research 2013-03, Vol.151 (3), p.446-450
Main Authors: Ward, Patrick, Connolly, Cathal, Murphy, Richard
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotechnology
Chromatography, Liquid
detection limit
Enzymes
Extraction processes
Life Sciences
Liquid chromatography
Mass Spectrometry
Microwaves
Nutrition
Oncology
Peptide Hydrolases - metabolism
Saccharomyces cerevisiae - chemistry
Saccharomyces cerevisiae - metabolism
selenomethionine
Selenomethionine - analysis
Selenomethionine - metabolism
Sonication
Trace elements
ultrasonic treatment
Yeast
Yeasts
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Summary:The purpose of this study was to reduce the extraction time, to hours instead of days, for quantification of the selenomethionine (SeMet) content of selenized yeast. An accelerated method using microwave-assisted enzymatic extraction and ultrasonication was optimized and applied to certified reference material (selenized yeast reference material (SELM)-1). Quantitation of SeMet in the extracts was performed by liquid chromatography with inductively coupled plasma mass spectrometry. The limits of detection and quantitation were 5 ppb SeMet and 15 ppb SeMet respectively and the signal response was linear up to 1,500 ppb SeMet. The average recovery of spiked SeMet from the selenized yeast matrix was 97.7 %. Analysis of an SELM-1 using this method resulted in 100.9 % recovery of the certified value (3448 ± 146 ppm SeMet). This method is suitable for fast reliable determination of SeMet in selenized yeast.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-012-9571-x