Alternative splicing of the nuclear progestin receptor in a perciform teleost generates novel mechanisms of dominant-negative transcriptional regulation

• Published in: General and comparative endocrinology 2013-02, Vol.182, p.24-40
• Main Authors: Zapater, Cinta, Chauvigné, François, Fernández-Gómez, Beatriz, Finn, Roderick Nigel, Cerdà, Joan
• Format: Article
• Language: English
• Subjects: Actinopterygii; alternative splicing; Alternative Splicing - genetics; Alternative Splicing - physiology; Animals; birds; cytoplasm; Dominant-negative inhibition; Endocrinology & Metabolism; Estradiol - metabolism; Estrogen; explants; Female; fish; Fish Proteins - genetics; Fish Proteins - metabolism; Follicle Stimulating Hormone - metabolism; follicle-stimulating hormone; FSH; gene expression; Gene Expression Regulation - genetics; Gene Expression Regulation - physiology; genes; Isoform; mammals; Marine; messenger RNA; Nuclear progestin receptor; oocytes; oogonia; ovarian follicles; Ovary; Ovary - metabolism; phylogeny; progestational hormones; Protein Isoforms - genetics; Protein Isoforms - metabolism; receptors; Receptors, Progesterone - genetics; Receptors, Progesterone - metabolism; reptiles; Sarcopterygii; Sea Bream - genetics; Sea Bream - metabolism; Sparus aurata; Teleost; Teleostei; transcription (genetics); vitellogenesis
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2012.11.015

Highlights ► Higher orders of Teleostei retain a single copy of the nuclear pgr gene. ► Four nuclear Pgr splice variants are coexpressed in the seabream ovary. ► Two Pgr isoforms show distinct mechanisms of dominant-negative inhibition. ► Pgr isoform expression is differentially regulated during ovarian development in vivo . ► FSH and E2 differentially regulate the expression of ovarian pgr isoforms in vitro.