Downregulation of CCR5 Expression on the Peripheral Blood CD8 super(+) T Cells of Southeastern Iranian Patients with Chronic Hepatitis B Infection

• Published in: Inflammation 2013-02, Vol.36 (1), p.136-140
• Main Authors: Ahmadabadi, Behzad Nasiri, Hassanshahi, Gholamhossein, Khoramdelazad, Hossein, Mirzaei, Vahid, Sajadi, Seyed Mohammad Ali, Hajghani, Masomeh, Khodadadi, Hassan, Pourali, Reza, Arababadi, Mohammad Kazemi, Kennedy, Derek
• Format: Article
• Language: English
• Subjects: CCL4 protein; CCR5 protein; CD8 antigen; Chronic infection; Cytotoxicity; Flow cytometry; Hepatitis B; Hepatitis B virus; Hepatocytes; Leukocyte migration; Liver; Lymphocytes T; Peripheral blood; Polymerase chain reaction
• ISSN: 0360-3997; 1573-2576
• DOI: 10.1007/s10753-012-9528-4

Studies indicated that CC receptor 5 (CCR5), as a receptor for CC ligand 3, CCL4, and CCL5, plays important roles in the recruitment of T cytotoxic lymphocytes to the liver of chronic HBV (CHB)-infected patients. The main purpose of this study was to investigate the expression levels of CCR5 on the CD8 super(+) T lymphocytes of CHB patients. This clinical study was performed on 63 CHB patients and 96 healthy controls. Flow cytometric analysis was performed to examine the expression of CCR5 on CD8 super(+) T cells of CHB patients. Real-time PCR was also used for HBV-DNA quantification. The results of our study demonstrated that CCR5 expressing T cytotoxic cells were decreased significantly in CHB patients in comparison to healthy control. Based on our results, it can be concluded that the percent of CCR5 super(+)/CD8 super(+) T cells in Iranian CHB patients is significantly decreased, hence their migration to the infected liver, and HBV eradication from the hepatocytes is disrupted.