The diversity of soil culturable fungi in the three alpine shrub grasslands of Eastern Qilian Mountains

• Published in: Frontiers of earth science 2013-03, Vol.7 (1), p.76-84
• Main Authors: ZHANG, Junzhong, MAN, Baiying, FU, Benzhong, LIU, Li, HAN, Changzhi
• Format: Article
• Language: English
• Subjects: Abundance; alpine shrub grasslands; Biodiversity; culturable fungi; diversity; Diversity indices; Earth and Environmental Science; Earth Sciences; Fungi; Genetic diversity; Grasslands; ITS序列; Mountains; rDNA ITS sequence analysis; Research Article; Rhododendron; Shannon-Wiener指数; Shrublands; Species diversity; 东祁连山; 多样性指数; 真菌培养; 草原土壤; 金露梅灌丛; 高山
• ISSN: 2095-0195; 2095-0209
• DOI: 10.1007/s11707-012-0345-8

To understand the diversity of culturable fungi in soil at alpine sites, Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland of the Eastern Qilian Mountains were selected to investigate. Three methods, including tradi- tional culturing, rDNA internal transcribed spacer （ITS） sequence analysis, and economical efficiency analysis, were carried out to estimate the diversity of soil culturable fungi of these three alpine shmblands. A total of 35 strains of culturable fungi were cultured by dilution plate technique and were analyzed by rDNA ITS sequence. The diversity indices such as species abundance （S）, Shannon-Wiener index （H）, Simpson dominance index （D）, and Pielou evenness index （3） of Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland were ranged between 16 and 17, 2.66-2.71, 0.92, 0.95~）.97 respectively. The results showed that the diversity of soil fungi were abundant in these three types of alpine shrub grasslands, while further study should be done to explore their potential value.