Overlapping transcription structure of human cytomegalovirus UL140 and UL141 genes

Transcription of human cytomegalovirus UL/b′ region has been studied extensively for some genes. In this study, transcripts of the UL140 and UL141, two of the UL/b′ genes, were identified in late RNAs of three HCMV isolates using Northern blot hybridization, cDNA library screening and RACE-PCR. At l...

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Bibliographic Details
Published in:Journal of biosciences 2013-03, Vol.38 (1), p.35-44
Main Authors: Ma, Yanping, Li, Mali, Zheng, Bo, Wang, Ning, Gao, Shuang, Wang, Lin, Qi, Ying, Sun, Zhengrong, Ruan, Qiang
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Blotting, Northern
cDNA libraries
Cell Biology
Cell Line
complementary DNA
Cytomegalovirus - genetics
Cytomegalovirus - isolation & purification
Cytomegalovirus Infections - virology
Deoxyribonucleic acid
DNA
Gene Expression Regulation, Viral
Gene Library
genes
Genomics
Human cytomegalovirus
Human herpesvirus 5
Humans
Hybridization
Infant
Life Sciences
Microbiology
Northern blotting
Open Reading Frames
Plant Sciences
RNA
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Messenger - metabolism
screening
Transcription, Genetic
Viral Proteins - genetics
Viral Proteins - metabolism
Viruses
Zoology
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Summary:Transcription of human cytomegalovirus UL/b′ region has been studied extensively for some genes. In this study, transcripts of the UL140 and UL141, two of the UL/b′ genes, were identified in late RNAs of three HCMV isolates using Northern blot hybridization, cDNA library screening and RACE-PCR. At least three transcripts with length of 2800, 2400 and 1700 nt, as well as a group of transcripts of about 1000–1300 nt, were found in this gene region with an accordant 3′ ends. Among the transcripts, two initiated upstream of the start code of the UL140 gene and contained the UL140 and UL141 open reading frame (ORF), one initiated in the middle of the UL140 gene, and could encode short ORFs upstream of the UL141 ORF. A group of transcripts initiated upstream or downstream of the start code of the UL141 gene, and could encode ‘nested’ ORFs, including the UL141 ORF. These ‘nested’ ORFs possess different initiation sites but the same termination site as that of the UL141 ORF.
ISSN:0250-5991
0973-7138
DOI:10.1007/s12038-012-9293-4