Soil fertility and establishment potential of inoculated cyanobacteria in rice crop grown under non-flooded conditions

• Published in: Paddy and water environment 2013, Vol.11 (1-4), p.175-183
• Main Authors: Prasanna, Radha, Sharma, Esha, Sharma, Pratima, Kumar, Arun, Kumar, Roshan, Gupta, Vishal, Pal, Ram Krishna, Shivay, Yashbir Singh, Nain, Lata
• Format: Article
• Language: English
• Subjects: Agriculture; Anabaena doliolum; Anabaena torulosa; Bacteria; Biomedical and Life Sciences; Cereal crops; Crops; Cyanobacteria; Decomposing organic matter; Deoxyribonucleic acid; DNA; Ecotoxicology; Fertilizers; Floods; Flora; Geoecology/Natural Processes; Humus; Hydrogeology; Hydrology/Water Resources; Life Sciences; Oryza sativa; Plant growth; Rice; Soil fertility; Soil Science & Conservation; Soils; Vermicomposting
• ISSN: 1611-2490; 1611-2504
• DOI: 10.1007/s10333-011-0302-2

An investigation was undertaken using a combination of microbiological and DNA-based approaches to evaluate combinations of two/three/four cyanobacterial strains (BF1 Anabaena torulosa ; BF2, Nostoc carneum ; BF3, Nostoc piscinale ; BF4, Anabaena doliolum) , using a novel vermicompost-based carrier, for their promise as inoculants in rice crop. The crop was maintained under 60% WHC through intermittent irrigation. Selected combinations (BF1 + 2 + 3; BF1 + 2 + 4; BF1 + 3 + 4) showed a consistent trend regarding their superiority over other treatments in terms of plant growth promotion and soil microbiological parameters. Microscopic examination of soil enrichment cultures raised from the treatments revealed their comparative abundance over native flora. A significant enhancement in terms of soil microbial biomass carbon and humus content was also recorded in these treatments. The soil enzymatic profile of the inoculated treatments involving FDA, dehydrogenase, acid phosphates, alkaline phosphatase, aryl esterase and β-glucosidase also revealed the promise of the inoculated strains. 16SrDNA sequencing, followed by BLAST analyses provided valuable information regarding the establishment of Anabaena strains. RFLP analyses of the 16SrDNA sequences of soil DNA and phylogenetic analyses vis a vis sequences of the inoculated cyanobacterial strains revealed the promise of treatments −BF1 + 2 + 3, BF1 + 2 + 4 and BF1 + 3 + 4. Panicle weight (yield attribute) was statistically at par with the fertilizer controls 1/2N + PK and significantly higher than 1/3N + PK. To our knowledge, this represents a first report on evaluating the establishment of cyanobacterial inoculants in rice crop grown under non-flooded conditions (or intermittent irrigation) using a combination of agronomic, microbiological and soil DNA related attributes.