Albirhodobacter marinus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from sea shore water of Visakhapatnam, India

• Published in: Antonie van Leeuwenhoek 2013-02, Vol.103 (2), p.347-355
• Main Authors: Nupur, Vaidya, Bhumika, Tanuku, Naga Radha Srinivas, Pinnaka, Anil Kumar
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Catalase - analysis; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty acids; Fatty Acids - analysis; Gram-negative bacteria; India; Life Sciences; Lipids; Medical Microbiology; Microbiology; Molecular Sequence Data; Original Paper; Ovatus; Oxidoreductases - analysis; Phylogenetics; Phylogeny; Plant Sciences; Quinones - analysis; Rhodobaca; Rhodobacter; Rhodobacter azotoformans; Rhodobacter sphaeroides; Rhodobacteraceae - classification; Rhodobacteraceae - genetics; Rhodobacteraceae - isolation & purification; Rhodobacteraceae - physiology; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Soil Science & Conservation; Taxonomy; Water analysis; Water sampling
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-012-9814-z

A novel marine, Gram-negative, rod-shaped bacterium, designated strain N9 T , was isolated from a water sample of the sea shore at Visakhapatnam, Andhra Pradesh (India). Strain N9 T was found to be positive for oxidase and catalase activities. The fatty acids were found to be dominated by C 16:0 , C 18:1 ω7c and summed in feature 3 (C 16:1 ω7c and/or C 16:1 ω6c ). Strain N9 T was determined to contain Q-10 as the major respiratory quinone and phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, two phospholipids and four unidentified lipids as polar lipids. The DNA G+C content of the strain N9 T was found to be 63 mol%. 16S rRNA gene sequence analysis indicated that Rhodobacter sphaeroides , Rhodobacter johrii , Pseudorhodobacter ferrugineus , Rhodobacter azotoformans , Rhodobacter ovatus and Pseudorhodobacter aquimaris were the nearest phylogenetic neighbours, with pair-wise sequence similarities of 95.43, 95.36, 94.24, 95.31, 95.60 and 94.74 %, respectively. Phylogenetic analysis showed that strain N9 T formed a distinct branch within the family Rhodobacteraceae and clustered with the clade comprising species of the genus Pseudorhodobacter , together with species of the genera Roseicitreum , Roseinatronobacter , Roseibaca and Rhodobaca . Species of the genus Pseudorhodobacter are phylogenetically close with a 16S rRNA gene sequence dissimilarity of 5.9–7.3 % (92.7–94.1 % similarity). Based on the above-mentioned phenotypic characteristics and on phylogenetic inference, strain N9 T is proposed as a representative of a new genus and a novel species of the family Rhodobacteraceae as Albirhodobacter marinus gen. nov., sp. nov. The type strain of Albirhodobacter marinus is N9 (= MTCC 11277 T  = JCM 17680 T ).