In vivo visualization of gold-loaded cells in mice using x-ray computed tomography

Abstract The ability to perform cell tracking using x-ray computed tomography combined with gold nanoparticles has been demonstrated recently on ex vivo samples using different malignant and nonmalignant cell lines. Here we proved the concept of the method for in vivo assessment in a small-animal mo...

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Bibliographic Details
Published in:Nanomedicine 2013-02, Vol.9 (2), p.284-292
Main Authors: Astolfo, Alberto, PhD, Schültke, Elisabeth, MD, PhD, Menk, Ralf Hendrik, PhD, Kirch, Robert D., PhD, Juurlink, Bernhard H.J., PhD, Hall, Christopher, PhD, Harsan, Laura-Adela, PhD, Stebel, Marco, MS, Barbetta, Davide, DVM, Tromba, Giuliana, PhD, Arfelli, Fulvia, PhD
Format: Article
Language:English
Subjects:
Animal models
Animals
Biodistribution
Brain - diagnostic imaging
Brain Neoplasms - diagnostic imaging
Brain tumors
Cell Line, Tumor
Computed tomography
Gold
Gold - analysis
Gold nanoparticles
High-resolution imaging
In vivo
Internal Medicine
Ionizing radiation
Male
Metal Nanoparticles - analysis
Mice
Mice, Nude
Monte Carlo Method
Monte Carlo simulation
nanoparticles
nanotechnology
Rats
spatial discrimination
Synchrotron radiation
Tomography, X-Ray Computed - methods
Tumor cells
X-ray computed tomography
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Summary:Abstract The ability to perform cell tracking using x-ray computed tomography combined with gold nanoparticles has been demonstrated recently on ex vivo samples using different malignant and nonmalignant cell lines. Here we proved the concept of the method for in vivo assessment in a small-animal model of malignant brain tumors. The limitations of the method due to radiation dose constraints were investigated using Monte Carlo simulations. Taking into consideration different x-ray entrance doses and the spatial resolution, the visibility of the cell clusters was evaluated. The results of the experiments conducted on mice implanted with F98 tumor cells confirmed the prediction of the Monte Carlo calculations. Small clusters of cells exogenously loaded with gold nanoparticles could be visualized using our in vivo method. From the Clinical Editor This article discusses the use of CT-based detection of gold nanoparticle loaded cells of interest in small-animal models of malignant brain tumors, where small clusters of cells loaded with gold nanoparticles could be visualized.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2012.06.004