Presence of Quorum-Sensing Genes in Pseudomonas isolates Infecting Cystic Fibrosis and Non-cystic Fibrosis Patients

Ninety-one Pseudomonas aeruginosa isolates recovered from cystic fibrosis and non-cystic fibrosis patients were evaluated regarding the ability to form biofilm and acyl-homoserine lactones production and for the presence of five quorum-sensing (QS) regulatory genes (lasI, lasR, rhlI, rhlR, and vfr)....

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Bibliographic Details
Published in:Current microbiology 2013-04, Vol.66 (4), p.418-420
Main Authors: Perez, Leandro Reus Rodrigues, Machado, Alice Beatriz Mombach Pinheiro, Barth, Afonso Luís
Format: Article
Language:English
Subjects:
Acyl-Butyrolactones - metabolism
Bacteria
Bacteriology
Biofilms
Biofilms - growth & development
Biomedical and Life Sciences
Biotechnology
Cystic fibrosis
Cystic Fibrosis - complications
fibrosis
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genomics
Humans
Life Sciences
Metabolic Networks and Pathways - genetics
Microbiology
patients
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - isolation & purification
Pseudomonas aeruginosa - physiology
Pseudomonas Infections - microbiology
Quorum Sensing
regulator genes
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Summary:Ninety-one Pseudomonas aeruginosa isolates recovered from cystic fibrosis and non-cystic fibrosis patients were evaluated regarding the ability to form biofilm and acyl-homoserine lactones production and for the presence of five quorum-sensing (QS) regulatory genes (lasI, lasR, rhlI, rhlR, and vfr). Most isolates (90.1 %) presented all five QS genes. Five isolates shown to be lasI/lasR-deficient were not able to produce biofilm in vitro. Moreover, one isolate harboring all five QS genes was also not able to form a biofilm. The function of rhlR gene may be compensated by the las QS system. However, in our study, all isolates which were deficient for the rhlR gene were also deficient for the lasI/lasR system. This may point to some hierarchy in QS regulation which may pose a potential for controlling biofilm infections due to P. aeruginosa.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-012-0290-5