Serum Vascular Adhesion Protein-1 correlates with vascular endothelial growth factor in patients with type II diabetes

• Published in: Journal of diabetes and its complications 2013-03, Vol.27 (2), p.162-166
• Main Authors: Yoshikawa, Nami, Noda, Kousuke, Shinoda, Hajime, Uchida, Atsuro, Ozawa, Yoko, Tsubota, Kazuo, Mashima, Yukihiko, Ishida, Susumu
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Amine Oxidase (Copper-Containing) - blood; Amine Oxidase (Copper-Containing) - chemistry; Cell adhesion & migration; Cell Adhesion Molecules - blood; Cell Adhesion Molecules - chemistry; Diabetes; Diabetes Mellitus, Type 2 - complications; Diabetic retinopathy; Diabetic Retinopathy - blood; Diabetic Retinopathy - physiopathology; Endocrinology & Metabolism; Enzyme-Linked Immunosorbent Assay; Epiretinal Membrane - blood; Epiretinal Membrane - complications; Female; Humans; Intercellular Adhesion Molecule-1 - blood; Leukocytes; Macular degeneration; Macular Degeneration - blood; Macular Degeneration - complications; Male; Middle Aged; Proteins; Retinal Perforations - blood; Retinal Perforations - complications; Semicarbazide-sensitive amine oxidase; Severity of Illness Index; Solubility; Up-Regulation; Vascular adhesion protein-1; Vascular Cell Adhesion Molecule-1 - blood; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - blood
• ISSN: 1056-8727; 1873-460X
• DOI: 10.1016/j.jdiacomp.2012.09.001

Abstract Aims To study serum levels of soluble vascular adhesion protein (sVAP)-1 in type II diabetic patients with retinopathy. Methods Serum samples were obtained from 53 consecutive patients, including 14 cases with non-angiogenic ocular diseases, i.e. , epiretinal membrane (ERM) and idiopathic macular hole (MH), 19 cases with age-related macular degeneration (AMD), and 20 cases with diabetic retinopathy (DR). Protein levels of sVAP-1, intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1, and vascular endothelial growth factor (VEGF) were determined by enzyme-linked immunosorbent assay. Enzymatic activity of semicarbazide-sensitive amine oxidase (SSAO) was also measured. Results Serum level of sVAP-1 showed a moderate correlation with SSAO activity in all cases. Patients with DR had higher levels of serum sVAP-1 than subjects with ERM and MH, or those with AMD; however, severity of DR is not related to the serum levels of sVAP-1. Serum sVAP-1 correlated positively with VEGF in patients with DR, but not in those with ERM and MH, or those with AMD. Neither soluble ICAM-1 nor VCAM-1 correlated with VEGF, even in subjects with DR. Conclusion The current data demonstrate the elevated serum levels of sVAP-1 and correlation between sVAP-1 and VEGF in patients with type II diabetes.