A novel microextraction by packed sorbent-gas chromatography procedure for the simultaneous analysis of antiepileptic drugs in human plasma and urine

• Published in: Journal of separation science 2012-11, Vol.35 (21), p.2970-2977
• Main Authors: Rani, Susheela, Malik, Ashok Kumar
• Format: Article
• Language: English
• Subjects: Alprazolam - blood; Alprazolam - isolation & purification; Alprazolam - urine; Analysis; Analytical chemistry; Anticonvulsants - analysis; Anticonvulsants - blood; Anticonvulsants - isolation & purification; Anticonvulsants - urine; Antiepileptics drugs; Biological and medical sciences; Carbamazepine - analogs & derivatives; Carbamazepine - blood; Carbamazepine - isolation & purification; Carbamazepine - urine; Chromatography; Drugs; Epilepsy; Epilepsy - blood; Epilepsy - drug therapy; Epilepsy - urine; Gas chromatography; Gas Chromatography-Mass Spectrometry - methods; General pharmacology; Human; Humans; Linearity; Medical sciences; Micro extraction by packed sorbent; Monitoring; Patients; Pharmacology; Pharmacology. Drug treatments; Phenytoin - blood; Phenytoin - isolation & purification; Phenytoin - urine; Plasma; Solid Phase Microextraction - methods; Urine
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201200439

A simple, accurate, and sensitive microextraction by packed sorbent–gas chromatography‐mass spectrometry method has been developed for the simultaneous quantification of four antiepileptic drugs; oxcarbazepine, carbamazepine, phenytoin, and alprazolam in human plasma and urine as a tool for drug monitoring. Caffeine was used as internal standards for the electron ionization mode. An original pretreatment procedure on biological samples, based on microextraction in packed syringe using C18 as packing material gave high extraction yields (69.92–99.38%), satisfactory precision (RSD < 4.7%) and good selectivity. Linearity was found in the 0.1–500 ng/mL range for these drugs with limits of detection (LODs) between 0.0018 and 0.0036 ng/mL. Therefore, the method has been found to be suitable for the therapeutic drug monitoring of patients treated with oxcarbazepine, carbamazepine, phenytoin, and alprazolam. After validation, the method was successfully applied to some plasma samples from patients undergoing therapy with one or more of these drugs. A comparison of the detection limit with similar methods indicates high sensitivity of the present method over the earlier reported methods. The present method is applied for the analysis of these drugs in the real urine and plasma samples of the epileptic patients.