Complete Identification of Proteins Responsible for Human Blood Plasma Fouling on Poly(ethylene glycol)-Based Surfaces

The resistance of poly(ethylene glycol) (PEG) against protein adsorption is crucial and has been widely utilized in various biomedical applications. In this work, the complete protein composition of biofilms deposited on PEG-based surfaces from human blood plasma (BP) was identified for the first ti...

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Bibliographic Details
Published in:Langmuir 2013-03, Vol.29 (10), p.3388-3397
Main Authors: Riedel, Tomáš, Riedelová-Reicheltová, Zuzana, Májek, Pavel, Rodriguez-Emmenegger, César, Houska, Milan, Dyr, Jan E, Brynda, Eduard
Format: Article
Language:English
Subjects:
Adsorption
Applied sciences
Chemistry
Chromatography, Liquid
Exact sciences and technology
General and physical chemistry
Humans
Organic polymers
Physicochemistry of polymers
Plasma - chemistry
Polyethylene Glycols - chemistry
Properties and characterization
Proteins - chemistry
Surface Properties
Tandem Mass Spectrometry
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Summary:The resistance of poly(ethylene glycol) (PEG) against protein adsorption is crucial and has been widely utilized in various biomedical applications. In this work, the complete protein composition of biofilms deposited on PEG-based surfaces from human blood plasma (BP) was identified for the first time using nanoLC-MS/MS, a powerful tool in protein analysis. The mass of deposited BP and the number of different proteins contained in the deposits on individual surfaces decreased in the order of self-assembling monolayers of oligo(ethylene glycol) alkanethiolates (SAM) > poly(ethylene glycol) end-grafted onto a SAM > poly(oligo(ethylene glycol) methacrylate) brushes prepared by surface initiated polymerization (poly(OEGMA)). The BP deposit on the poly(OEGMA) surface was composed only of apolipoprotein A-I, apolipoprotein B-100, complement C3, complement C4-A, complement C4-B, histidine-rich glycoprotein, Ig mu chain C region, fibrinogen (Fbg), and serum albumin (HSA). The total resistance of the surface to the Fbg and HSA adsorption from single protein solutions suggested that their deposition from BP was mediated by some of the other proteins. Current theories of protein resistance are not sufficient to explain the observed plasma fouling. The research focused on the identified proteins, and the experimental approach used in this work can provide the basis for the understanding and rational design of plasma-resistant surfaces.
ISSN:0743-7463
1520-5827
DOI:10.1021/la304886r