Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2013-05, Vol.105, p.230-235
• Main Authors: Jovanović, Željka, Radosavljević, Aleksandra, Kačarević-Popović, Zorica, Stojkovska, Jasmina, Perić-Grujić, Aleksandra, Ristić, Mirjana, Matić, Ivana Z., Juranić, Zorica D., Obradovic, Bojana, Mišković-Stanković, Vesna
• Format: Article
• Language: English
• Subjects: Biomimetic Materials - metabolism; Bioreactor conditions; Bioreactors; Body Fluids - chemistry; Body Fluids - metabolism; Cell Proliferation - drug effects; Cells, Cultured; HeLa Cells; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry; In vitro cytotoxicity; Leukocytes, Mononuclear - drug effects; Materials Testing; Metal Nanoparticles - chemistry; Nanocomposites - chemistry; Poly(N-vinyl-2-pyrrolidone); Polyvinyls - chemistry; Pyrrolidines - chemistry; Silver - chemistry; Silver - metabolism; Silver nanoparticles; Silver release kinetics
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2012.12.055

[Display omitted] ► Ag/PVP nanocomposites with incorporated AgNPs obtained by gamma irradiation. ► Dose-dependent cytotoxicity of AgNPs and Ag+ ions against PBMC. ► Diffusion: a dominant mechanism of silver release in all applied conditions. ► Ag/PVP nanocomposites optimization for wound dressings or soft tissue implants. Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using γ-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MTT assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37°C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4μmoldm−3, with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants.