Development, characterisation and biocompatibility testing of a cobalt-containing titanium phosphate-based glass for engineering of vascularized hard tissues

• Published in: Materials Science & Engineering C 2013-05, Vol.33 (4), p.2104-2112
• Main Authors: Lee, In-Ho, Yu, Hye-sun, Lakhkar, Nilay J., Kim, Hae-Won, Gong, Myoung-Seon, Knowles, Jonathan C., Wall, Ivan B.
• Format: Article
• Language: English
• Subjects: Animals; Biocompatibility; Cell Line, Tumor; Cell Proliferation - drug effects; Cobalt - pharmacology; Glass - chemistry; Humans; Hydrogen-Ion Concentration - drug effects; Ions; Materials characterisation; Materials Testing - methods; Neovascularization, Physiologic - drug effects; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - metabolism; Phosphate glass; Rats; Spectroscopy, Fourier Transform Infrared; Tissue engineering; Tissue Engineering - methods; Titanium - pharmacology; Transition Temperature - drug effects; Vascular Endothelial Growth Factor A - secretion; X-Ray Diffraction
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2013.01.024

There is a continuing need to develop scaffold materials that can promote vascularisation throughout the tissue engineered construct. This study investigated the effect of cobalt oxide (CoO) doped into titanium phosphate glasses on material properties, biocompatibility and vascular endothelial growth factor (VEGF) secretion by osteoblastic MG63 cells. Glasses composed of (P2O5)45(Na2O)20(TiO2)05(CaO)30−x(CoO)x(x=0, 5, 10, and 15mol%) were fabricated and the effect of Co on physicochemical properties including density, glass transition temperature (Tg), degradation rate, ion release, and pH changes was assessed. The results showed that incorporation of CoO into the glass system produced an increase in density with little change in Tg. It was then confirmed that the pH did not change significantly when CoO was incorporated in the glass, and stayed constant at around 6.5–7.0 throughout the dissolution study period of 336h. Ion release results followed a specific pattern with increasing amounts of CoO. In general, although incorporation of CoO into a titanium phosphate glass increased its density, other bulk and surface properties of the glass did not show any significant changes. Cell culture studies performed using MG63 cells over a 7-day period indicated that the glasses provide a stable surface for cell attachment and are biocompatible. Furthermore, VEGF secretion was significantly enhanced on all glasses compared with standard tissue culture plastic and Co doping enhanced this effect further. In conclusion, the developed Co-doped glasses are stable and biocompatible and thus offer enhanced potential for engineering vascularized tissue. ► Phosphate-based glasses can be successfully doped with cobalt oxide. ► The resulting glass is highly stable with low degradation rate. ► Co-doped glasses are biocompatible but do not favour cell proliferation. ► Osteoblastic MG63 cells upregulate production of VEGF in response to Co-doped glass.