Efficient conversion of high concentration of glycerol to Monacolin K by solid-state fermentation of Monascus purpureus using bagasse as carrier

• Published in: Bioprocess and biosystems engineering 2013-03, Vol.36 (3), p.293-299
• Main Authors: Lu, Li-Ping, Zhang, Bo-Bo, Xu, Gan-Rong
• Format: Article
• Language: English
• Subjects: Bagasse; Biodiesel fuels; Bioengineering; bioprocessing; Biotechnology; Biotechnology - methods; carbon; Carbon sources; Cellulose - chemistry; chemical concentration; Chemistry; Chemistry and Materials Science; Comparative studies; Crop residues; engineering; Environmental Engineering/Biotechnology; Environmental Pollutants; Fermentation; Food Science; glycerol; Glycerol - chemistry; Glycine max - chemistry; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; inoculum; lovastatin; Lovastatin - chemistry; Metabolites; Moisture content; Monascus - metabolism; Monascus purpureus; nitrogen; Nitrogen - chemistry; Original Paper; Particle Size; solid state fermentation; soybean meal; Soybeans; submerged fermentation; water content
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-012-0784-3

High concentration of glycerol was used as the sole carbon source for efficient production of Monacolin K (MK) by solid-state fermentation (SSF) of Monascus purpureus 9901 using agricultural residue (bagasse), as an inert carrier. A comparative study showed that MK production in SSF was about 5.5 times higher than that of submerged fermentation when 26 % of glycerol was used, which may be due to the formation of glycerol concentration gradients in the inert carrier and less catabolite repression in SSF. For enhancement of MK yield in SSF, the effects of different influential variables, such as glycerol concentration, nitrogen source and its concentration, initial moisture content, inoculum size and particle size of bagasse, were systematically examined. All the factors mentioned above had an effect on the MK production in SSF to some extent. The maximal yield of MK (12.9 mg/g) was achieved with 26 % glycerol, 5 % soybean meal, 51 % initial moisture content, 20 % inoculum size and 1 mm particle size of bagasse. The results in this study may expand our understanding on the application of SSF using agricultural residue as carrier for production of useful microbial metabolites, especially the efficient conversion of high concentration of glycerol to MK by Monascus purpureus.