Microbial characteristics of a CANON reactor during the start-up period seeding conventional activated sludge

An up-flow oxygen-controlled biofilm reactor filled with volcanic filter was used for a completely autotrophic nitrogen-removal over nitrite (CANON) process. The reactor was successfully established by seeding conventional activated sludge at ambient temperature without additional biomass inoculatio...

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Bibliographic Details
Published in:Water science and technology 2013-01, Vol.67 (3), p.635-643
Main Authors: TAO LIU, DONG LI, ZENG, Hui-Ping, CHANG, Xiao-Yan, JIE ZHANG
Format: Article
Language:English
Subjects:
Activated sludge
Ambient temperature
Ammonium
Ammonium compounds
Analytical methods
Applied sciences
Bacteria
Biofilms
Bioreactors
Bioreactors - microbiology
Communities
Community structure
Denaturing Gradient Gel Electrophoresis
Exact sciences and technology
Gels
Gene Library
General purification processes
In Situ Hybridization, Fluorescence
Inoculation
Mathematical morphology
Microbial Consortia
Microorganisms
Microscopy, Electron, Scanning
Niches
Nitrobacter
Nitrobacter - metabolism
Nitrogen
Nitrogen - metabolism
Nitrogen removal
Nitrosomonas
Nitrosomonas europaea - metabolism
Nucleation
Oxidation
Phylogeny
Pollution
Profiles
Reactors
Removal
Sewage - microbiology
Sludge
Spatial distribution
Wastewaters
Water treatment and pollution
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Description
Summary:An up-flow oxygen-controlled biofilm reactor filled with volcanic filter was used for a completely autotrophic nitrogen-removal over nitrite (CANON) process. The reactor was successfully established by seeding conventional activated sludge at ambient temperature without additional biomass inoculation. An average total nitrogen (TN) removal rate of 1.1 kg·(m(3) d)(-1) was achieved after 180 days' operation. The bacterial morphology, community structure and spatial distribution of nitrogen removal microorganisms were analyzed by using some molecular biotechniques. Denaturant gel gradient electrophoresis (DGGE) profiles showed a distinct community shift of nitrite oxidizing bacteria (NOB) during the start-up period, which was not the same as that of aerobic ammonium-oxidizing bacteria (AerAOB) or anaerobic ammonium-oxidizing bacteria (AnAOB). Phylogenetic results indicated the predominance of Nitrosomonas, 'Candidatus Brocadia fulgida' and Nitrobacter for nitrogen removal in the system, all of which coexisted without a distinguishable niche on the biofilm.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2012.610