Simultaneous determination for oxicam non-steroidal anti-inflammatory drugs in human serum by liquid chromatography–tandem mass spectrometry

Abstract A high-performance liquid chromatography–tandem mass spectrometry (LC/MS/MS) technique was developed for the simultaneous determination of five non-steroidal anti-inflammatory oxicam drugs (ampiroxicam, tenoxicam, piroxicam, meloxicam and lornoxicam) in human plasma. These five oxicam drugs...

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Bibliographic Details
Published in:Forensic science international 2013-04, Vol.227 (1), p.100-102
Main Authors: Shirako, Junichi, Kawasaki, Marina, Komine, Kotoe, Kunisue, Yoko, Terada, Masaru, Sasaki, Chizuko, Irie, Wataru, Murakami, Chikako, Tonooka, Keiko, Tomobe, Koji, Shinozuka, Tatsuo
Format: Article
Language:English
Subjects:
Analysis
Analytical chemistry
Anti-Inflammatory Agents, Non-Steroidal - blood
Anti-Inflammatory Agents, Non-Steroidal - chemistry
Chromatography
Chromatography, Liquid - methods
Forensic sciences
Forensic Toxicology - methods
Humans
LC/MS/MS
Mass spectrometry
Molecular Structure
NSAIDs
Oxicam derivatives
Pathology
Piroxicam - analogs & derivatives
Piroxicam - blood
Piroxicam - chemistry
Plasma
Solid phase extraction
Tandem Mass Spectrometry - methods
Thiazines - blood
Thiazines - chemistry
Thiazoles - blood
Thiazoles - chemistry
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Summary:Abstract A high-performance liquid chromatography–tandem mass spectrometry (LC/MS/MS) technique was developed for the simultaneous determination of five non-steroidal anti-inflammatory oxicam drugs (ampiroxicam, tenoxicam, piroxicam, meloxicam and lornoxicam) in human plasma. These five oxicam drugs and isoxicam (internal standard) were extracted from human plasma with an Oasis® MAX cartridge column and analysed on a Unison UK-C18 column (2.0 mm × 100 mm, 3 μm) with an acetonitrile:10 mM formic ammonium buffer (pH 3.0) (50:50) mobile phase at 0.20 ml/min at 37 °C. The analytes were detected using a tandem mass spectrometer, equipped with an electrospray ion source (ESI). The instrument was used in multiple-reaction-monitoring (MRM) mode. The extraction yields from a 200 μl human plasma sample (containing 10 ng of each drugs) with the Oasis® MAX cartridge column were 93.3–102.5%. The detection limits were 0.01–6.5 ng/ml (S/N = 3). Our developed method is very useful for the simultaneous determination of five oxicam (non-steroidal anti-inflammatory) drugs in human plasma by LC/MS/MS.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2012.11.016