Transcriptome analysis of Chlamydomonas reinhardtii during the process of lipid accumulation

Algae are potential candidates for biodiesel production; thus, it is important to gain insight into the molecular mechanism of their lipid metabolism. Time-course transcriptome analyses were carried out during the lipid biosynthesis and accumulation processes of the model green alga Chlamydomonas re...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2013-04, Vol.101 (4), p.229-237
Main Authors: Lv, Hexin, Qu, Ge, Qi, Xizhen, Lu, Lina, Tian, Chaoguang, Ma, Yanhe
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
Acyltransferases - metabolism
Biodiesel
biosynthesis
Chlamydomonas
Chlamydomonas reinhardtii
Chlamydomonas reinhardtii - genetics
Chlamydomonas reinhardtii - metabolism
diacylglycerol acyltransferase
Diacylglycerol O-Acyltransferase - genetics
Diacylglycerol O-Acyltransferase - metabolism
economics
engineering
Gene Expression Profiling
gene expression regulation
Gene Expression Regulation, Plant
genes
Lipid Metabolism
Lipids
microRNA
Plant Proteins - genetics
Plant Proteins - metabolism
RNA, Small Interfering
RNA-seq
transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Transcriptome
transcriptomics
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Summary:Algae are potential candidates for biodiesel production; thus, it is important to gain insight into the molecular mechanism of their lipid metabolism. Time-course transcriptome analyses were carried out during the lipid biosynthesis and accumulation processes of the model green alga Chlamydomonas reinhardtii using the Illumina RNA-seq platform. Transcriptome results indicated that over 2500 genes are upregulated or during lipid accumulation compared to log phase growth. As a proof of principle, two of the enzymes required for lipid metabolism that were significantly up-regulated during lipid accumulation, Lyso-Phosphatidic Acid Acyltransferase (LPAAT), diacylglycerol acyltransferase (DAGAT) were knocked down using artificial microRNAs. Neutral lipid production decreased in strains knocked down in expression of the lpaat and dagat genes. In addition, forty-one transcription factors were up- or down-regulated during the lipid accumulation process. This transcriptome data will be useful for engineering economic algae species aimed at biodiesel production. ► We profile the gene expression during the lipid accumulation by RNA-seq analysis. ► Lipid production decreased in LPAAT and DAGAT knockdown strain. ► Fatty acid biosynthesis and TAG assembly affected by PDH and G3PDH expression
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2013.01.004