In Vivo Profiling and Visualization of Cellular Protein-Lipid Interactions Using Bifunctional Fatty Acids

Bifunctional lipid technology: Cells convert externally added photoactivatable and “clickable” fatty acids into a variety of bifunctional phospholipids that can be covalently linked to their protein‐binding partners by irradiation with UV light. Derivatization of the clickable group with a reporter...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2013-04, Vol.52 (14), p.4033-4038
Main Authors: Haberkant, Per, Raijmakers, Reinout, Wildwater, Marjolein, Sachsenheimer, Timo, Brügger, Britta, Maeda, Kenji, Houweling, Martin, Gavin, Anne-Claude, Schultz, Carsten, van Meer, Gerrit, Heck, Albert J. R., Holthuis, Joost C. M.
Format: Article
Language:English
Subjects:
Animals
cell imaging
CHO Cells
Click Chemistry
Cricetinae
Cricetulus
Fatty Acids - chemical synthesis
Fatty Acids - chemistry
Fatty Acids - metabolism
HeLa Cells
Humans
lipids
Microscopy, Confocal
Phospholipids - chemistry
Phospholipids - metabolism
photoaffinity labeling
Protein Binding
proteins
Proteins - chemistry
Proteins - metabolism
Ultraviolet Rays
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Summary:Bifunctional lipid technology: Cells convert externally added photoactivatable and “clickable” fatty acids into a variety of bifunctional phospholipids that can be covalently linked to their protein‐binding partners by irradiation with UV light. Derivatization of the clickable group with a reporter molecule makes it possible to identify and image the lipid‐bound proteins in situ (see scheme).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201210178