Poly[tri(ethylene glycol) ethyl ether methacrylate]-Coated Surfaces for Controlled Fibroblasts Culturing

• Published in: ACS applied materials & interfaces 2013-03, Vol.5 (6), p.2197-2207
• Main Authors: Dworak, Andrzej, Utrata-Wesołek, Alicja, Szweda, Dawid, Kowalczuk, Agnieszka, Trzebicka, Barbara, Anioł, Jacek, Sieroń, Aleksander L, Klama-Baryła, Agnieszka, Kawecki, Marek
• Format: Article
• Language: English
• Subjects: Cell Adhesion; Cell Culture Techniques - methods; Cells, Cultured; Chromatography, Gel; Fibroblasts - cytology; Humans; Microscopy, Atomic Force; Microscopy, Fluorescence; Photoelectron Spectroscopy; Polyethylene Glycols - chemistry; Polymethacrylic Acids - chemistry
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/am3031882

Well-defined thermosensitive poly[tri(ethylene glycol) monoethyl ether methacrylate] (P(TEGMA-EE)) brushes were synthesized on a solid substrate by the surface-initiated atom transfer radical polymerization of TEGMA-EE. The polymerization reaction was initiated by 2-bromo-2-methylpropionate groups immobilized on the surface of the wafers. The changes in the surface composition, morphology, philicity, and thickness that occurred at each step of wafer functionalization confirmed that all surface modification procedures were successful. Both the successful modification of the surface and bonding of the P(TEGMA-EE) layer were confirmed by X-ray photoelectron spectroscopy (XPS) measurements. The thickness of the obtained P(TEGMA-EE) layers increased with increasing polymerization time. The increase of environmental temperature above the cloud point temperature of P(TEGMA-EE) caused the changes of surface philicity. A simultaneous decrease in the polymer layer thickness confirmed the thermosensitive properties of these P(TEGMA-EE) layers. The thermosensitive polymer surfaces obtained were evaluated for the growth and harvesting of human fibroblasts (basic skin cells). At 37 °C, seeded cells adhered to and spread well onto the P(TEGMA-EE)-coated surfaces. A confluent cell sheet was formed within 24 h of cell culture. Lowering the temperature to an optimal value of 17.5 °C (below the cloud point temperature of the polymer, T CP, in cell culture medium) led to the separation of the fibroblast sheet from the polymer layer. These promising results indicate that the surfaces produced may successfully be used as substrate for engineering of skin tissue, especially for delivering cell sheets in the treatment of burns and slow-healing wounds.