Participation of phosphatidylinositol-3'-kinase in signal transduction through galactosyl-containing glycoprotein receptors of segmentonuclear leukocytes under type 1 diabetes mellitus

It is shown that reduction of beta,D-galactosyl-containing carbohydrate determinants ofglycoconjugates on theplasmatic membrane of segmentonuclear neutrophills of peripheral blood under type 1 diabetes mellitus (DM) is correlated with changes in aggregation of these cells and may cause their functio...

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Published in:Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) Ukraine : 1994), 2012, Vol.58 (6), p.9-22
Main Authors: Sybirna, N O, Brodiak, I V, Bars'ka, M L, Vovk, O I
Format: Article
Language:Ukrainian
Subjects:
Androstadienes - pharmacology
Case-Control Studies
Cell Aggregation - drug effects
Cell Membrane - drug effects
Cell Membrane - metabolism
Cells, Cultured
Class Ia Phosphatidylinositol 3-Kinase - metabolism
Cytosol - drug effects
Cytosol - metabolism
Diabetes Mellitus, Type 1 - metabolism
Diabetes Mellitus, Type 1 - pathology
Humans
Neutrophil Activation - drug effects
Neutrophils - drug effects
Neutrophils - metabolism
Neutrophils - pathology
Plant Lectins - pharmacology
Protein Kinase Inhibitors - pharmacology
Protein Transport - drug effects
Receptors, Mitogen - chemistry
Receptors, Mitogen - metabolism
Signal Transduction - drug effects
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Summary:It is shown that reduction of beta,D-galactosyl-containing carbohydrate determinants ofglycoconjugates on theplasmatic membrane of segmentonuclear neutrophills of peripheral blood under type 1 diabetes mellitus (DM) is correlated with changes in aggregation of these cells and may cause their functional disorder. Changes in the parameters of ricin-induced neutrophil activation after inhibition of the phosphatidylinositol-3'-kinase (PI-3'-kinase) enzyme with wortmannin indicated that the functional state ofpolymorphonuclear leukocytes is mediated by signaling pathways in which PI-3'-kinase is involved. Thus, PI-3'-kinase-dependent signal networks are involved in the processes of signal transduction through galactosyl-containing glycoprotein receptors into neutrophilic leukocytes. Inertness of the intensity formation in time ofneutrophil granulocyte cell response on RCA-induced translocation of the p85alpha regulatory subunit of PI-3'-kinase from the cytosolic to the membrane fraction under type 1 DM is a consequence of changes in the number or structure of plasmatic galactosyl-containing glycoprotein receptors. The revealed changes may be etiologic premise of diabetic complications and chronic diseases that impair the functional condition of patients with type 1 DM.
ISSN:2522-9028