Sequencing, phylogenetic analysis, and potential recombination events of infectious bronchitis viruses isolated in Korea

The S2 glycoprotein and membrane (M) protein genes and S1 glycoprotein and nucleocapsid (N) genes of 11 Korean infectious bronchitis virus (IBV) isolates were amplified by RT-PCR, cloned, and sequenced. The resultant nucleotide sequences were compared with the published sequences for non-Korean IBV...

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Bibliographic Details
Published in:Virus genes 2013-04, Vol.46 (2), p.371-374
Main Authors: Song, Jae Eun, Jeong, Won Gu, Sung, Haan Woo, Kwon, Hyuk Moo
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biomedical and Life Sciences
Biomedicine
Chickens
Coronavirus Infections - veterinary
Coronavirus Infections - virology
genes
glycoproteins
Glycoproteins - genetics
infectious bronchitis
Infectious bronchitis virus
Infectious bronchitis virus - classification
Infectious bronchitis virus - genetics
Infectious bronchitis virus - isolation & purification
Medical Microbiology
membrane glycoproteins
Molecular Sequence Data
Nucleocapsid Proteins - genetics
nucleotide sequences
Phylogeny
Plant Sciences
Poultry Diseases - virology
Recombination, Genetic
Republic of Korea
Sequence Analysis, DNA
serotypes
Viral Envelope Proteins - genetics
Virology
viruses
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Summary:The S2 glycoprotein and membrane (M) protein genes and S1 glycoprotein and nucleocapsid (N) genes of 11 Korean infectious bronchitis virus (IBV) isolates were amplified by RT-PCR, cloned, and sequenced. The resultant nucleotide sequences were compared with the published sequences for non-Korean IBV strains. Korean IBV isolates formed two independent subclusters within the phylogenetic tree based on S2 glycoprotein gene sequences. However, four and two different clusters were formed in the phylogenetic tree based on S1 glycoprotein and M gene sequences, respectively. In particular, Korean IBV K446-01 and K203-02 strains appeared to be the result of recombination between an indigenous Korean IBV strains and a vaccine strain (Massachusetts serotype) currently used in Korea. The recent IBV isolate, K026-10, formed a new subgroup that was closely related to traditional Korean IBV group in a phylogenetic tree based on the S1 and S2 genes, but it was grouped into the traditional Korean IBV cluster in a phylogenetic tree based on the M and N genes. Our data show that field IBVs in Korea are continuing to evolve and that vaccine strains might actually play a critical role in the appearance of new IBV strains via recombination in the field.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-012-0856-0