Biocompatible nanostructured magnesium oxide-chitosan platform for genosensing application

• Published in: Biosensors & bioelectronics 2013-07, Vol.45, p.181-188
• Main Authors: Patel, Manoj Kumar, Ali, Md. Azahar, Zafaryab, Md, Agrawal, Ved Varun, Rizvi, M. Moshahid Alam, Ansari, Z.A., Ansari, S.G., Malhotra, Bansi D.
• Format: Article
• Language: English
• Subjects: Biocompatibility; Biofunctionalization; Biological and medical sciences; Biosensing Techniques - methods; Biosensors; Biotechnology; Chitosan - chemistry; DNA, Single-Stranded - isolation & purification; Electrochemistry; Fundamental and applied biological sciences. Psychology; Genosensor; Humans; Indium - chemistry; Limit of Detection; Magnesium Oxide - chemistry; Methods. Procedures. Technologies; Nanostructured MgO-Chitosan; Nanostructures - chemistry; Spectroscopy, Fourier Transform Infrared; Tin Compounds - chemistry; Various methods and equipments; Vibrio cholerae; Vibrio cholerae - isolation & purification; Vibrio cholerae - pathogenicity; X-Ray Diffraction
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2012.12.055

A novel organic–inorganic platform comprising of chitosan (CH) modified nanostructured magnesium oxide (nanoMgO) has been electrophoretically deposited on the indium-tin-oxide (ITO) substrate. The single stranded probe DNA (ssDNA) sequence of Vibrio cholerae has been covalently functionalized onto CH-nanoMgO/ITO surface. The cytotoxicity assay of nanoMgO particles, examined using human intestinal cell line (INT 407), reveals no significant cytotoxicity at the given doses in the range of 50–350μg/mL. The X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and various microscopic techniques have been employed for the structural and morphological analysis of the fabricated electrodes. The electrochemical response studies of ssDNA and fragmented genomic DNA hybridized electrode (dsGDNA/CH-nanoMgO/ITO) have been carried out using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The dsGDNA/CH-nanoMgO/ITO bioelectrode exhibits a linear response in the range 100–500ng/μL with improved sensitivity of 36.72nA/ng/cm2, faster response time of 3s and high stability of 3–4 months under refrigerated condition. The lower detection limit of fabricated genosensor has been estimated as 35.20ng/μL and it shows good reproducibility/repeatability. ► Chitosan modified nanostructured magnesium oxide biomaterial based genosensor is presented. ► Biocompatibility of nanoMgO confirmed by MTT assay using human intestinal cell line (INT 407). ► High sensitivity, fast response time, low detection limit and high stability found.