Acute aerocystitis in Piaractus mesopotamicus: Participation of eicosanoids and pro-inflammatory cytokines

• Published in: Fish & shellfish immunology 2013-05, Vol.34 (5), p.1057-1062
• Main Authors: Claudiano, Gustavo da Silva, Petrillo, Thalita R., Manrique, Wilson G., Castro, Marcello P., Loureiro, Bruna A., Marcusso, Paulo F., Belo, Marco A.A., Moraes, Julieta R.E., de Moraes, Flávio Ruas
• Format: Article
• Language: English
• Subjects: Aeromonas hydrophila - immunology; Animals; Anti-Inflammatory Agents - administration & dosage; Bacterial Vaccines - administration & dosage; Capillary Permeability; Cellular Structures; Characidae - immunology; Characidae - microbiology; Chemical mediators; Cytokines - metabolism; Dexamethasone - administration & dosage; Dose-Response Relationship, Drug; Eicosanoids - metabolism; Evans blue; Gram-Negative Bacterial Infections - immunology; Gram-Negative Bacterial Infections - metabolism; Gram-Negative Bacterial Infections - veterinary; Indomethacin - administration & dosage; Inflammation; Teleost; Thiazines - administration & dosage; Thiazoles - administration & dosage; Time Factors; Vaccines, Inactivated - administration & dosage; Vascular alteration
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2013.01.006

A total of 360 pacus (Piaractus mesopotamicus) were used to study vascular permeability (VP) and inflammatory cell component (CC) in induced aerocystitis in P. mesopotamicus through inoculation of inactivated Aeromonas hydrophila, and the effect of steroidal and nonsteroidal anti-inflammatory drugs. It was observed that after inoculation of A. hydrophila, the maximum VP occurred 180 min post-stimulus (MPS). Pretreatment with anti-inflammatory drugs inhibited VP, and the inhibitory effect of dexamethasone was seen earlier than the effects caused by meloxicam and indomethacin. Inoculation of the bacterium caused a gradual increase in the accumulation of cells, which reached a maximum 24 h post-stimulus (HPS). Pretreatment with dexamethasone, indomethacin and meloxicam reduced the accumulation of lymphocytes, thrombocytes, granulocytes and macrophages. There was no significant difference between the different doses of the drugs tested. The results suggest that eicosanoids and pro-inflammatory cytokines participate in chemical mediation in acute inflammation in pacus.