Hypolipidemic and antioxidant activities of polysaccharides from Rosae Laevigatae Fructus in rats

• Published in: Carbohydrate polymers 2013-04, Vol.94 (1), p.56-62
• Main Authors: Yu, Chen-Huan, Dai, Xiao-Yan, Chen, Qin, Zang, Jia-Na, Deng, Li-Li, Liu, Yue-Huan, Ying, Hua-Zhong
• Format: Article
• Language: English
• Subjects: Animals; antioxidant activity; antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Applied sciences; Biological and medical sciences; blood serum; Characterization; Drug Evaluation, Preclinical; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; Exact sciences and technology; Fruit - chemistry; galactose; Gene Expression - genetics; General and cellular metabolism. Vitamins; glucans; Glutathione Peroxidase - metabolism; Hydrogen Bonding; Hyperlipidemia; Hyperlipidemias - drug therapy; Hypolipidemic Agents - chemistry; Hypolipidemic Agents - isolation & purification; Hypolipidemic Agents - pharmacology; lipid metabolism; Lipid Metabolism - drug effects; Lipid Metabolism - genetics; Lipoprotein lipase; Lipoprotein Lipase - genetics; Lipoprotein Lipase - metabolism; Liver - drug effects; Liver - metabolism; Liver - pathology; Lovastatin - pharmacology; low density lipoprotein cholesterol; Male; Malondialdehyde - metabolism; mannose; Medical sciences; Molecular Weight; Natural polymers; oral administration; Peroxisome proliferator-activated receptor; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; PPAR gamma - genetics; PPAR gamma - metabolism; Rats; Rats, Sprague-Dawley; Rosaceae - chemistry; Starch and polysaccharides; Superoxide Dismutase - metabolism; triacylglycerols; xylose
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.01.006

► Two polysaccharides (RLP-1 and RLP-2) were purified from Rosae Laevigatae Fructus. ► RLP-1 was composed of Xyl, Man and Gal while RLP-2 was only composed of Glu. ► RLP-1 ameliorated high fat diet-induced hyperlipidemia in rats but RLP-2 could not. ► RLP-1 improved lipid profiles through regulating PPAR-γ/LPL pathway. Two major fractions (RLP-1 and RLP-2) were obtained by purifying the crude polysaccharides extracted from a traditional Chinese herb Rosae Laevigatae Fructus. The average molecular weight of RLP-1 and RLP-2 was 21.5kDa and 16.1kDa, respectively. Monosaccharide analysis indicated that RLP-1 was composed of xylose, mannose and galactose in the molar ratio of 1:11:8, while RLP-2 was only a glucan. Oral administration of RLP-1 could significantly decrease levels of serum total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C), inhibit hepatic lipid accumulation, increase antioxidant lipids and up-regulate expressions of peroxisome proliferator-activated receptor-γ (PPAR-γ) and lipoprotein lipase (LPL) in hyperlipidemia rats. These results suggest that RLP-1 improve hyperlipidemia possibly through regulating PPAR-mediated lipid metabolism. Therefore, could be explored as a possible agent for hyperlipidemia.