Antimicrobial electrospun membranes of chitosan/poly(ethylene oxide) incorporating poly(hexamethylene biguanide) hydrochloride

• Published in: Carbohydrate polymers 2013-04, Vol.94 (1), p.364-371
• Main Authors: Dilamian, M., Montazer, M., Masoumi, J.
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; anti-infective properties; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antimicrobial activity; Applied sciences; bacteria; Biguanides - chemistry; Biguanides - pharmacology; biocompatible materials; Biological and medical sciences; Calorimetry, Differential Scanning; Carbohydrate Conformation; Chitosan; Chitosan - chemistry; differential scanning calorimetry; Disk Diffusion Antimicrobial Tests; Electrochemical Techniques; Electrospinning; Escherichia coli; Escherichia coli - drug effects; Exact sciences and technology; Exchange resins and membranes; Forms of application and semi-finished materials; Fourier transform infrared spectroscopy; Kinetics; Medical sciences; Membranes, Artificial; Nanocomposites - chemistry; Nanocomposites - ultrastructure; Nanofiber; nanofibers; Nanofibers - chemistry; Nanofibers - ultrastructure; Pharmacology. Drug treatments; Poly(hexamethylene biguanide) hydrochloride (PHMB); polyethylene glycol; Polyethylene Glycols - chemistry; Polymer industry, paints, wood; Porosity; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus; Staphylococcus aureus - drug effects; Technology of polymers; thermal properties; Viscosity
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.01.059

► Introducing novel chitosan/PHMB nanofibers with improved characteristics. ► Enhanced antibacterial properties of chitosan with adding PHMB. ► Producing easier and finner chitosan nanofiber by adding PHMB. ► Increasing medical application of chitosan nanofibers. Here, antimicrobial nanofibrous membranes were produced by electrospinning of chitosan/poly(ethylene oxide) (PEO) solution in the presence of poly(hexamethylene biguanide) hydrochloride (PHMB). The influence of PHMB on the electrospinnability and antimicrobial properties of chitosan/PEO nanofibers were studied. Further, viscosity of the solutions as well as morphology of the nanofibrous structures were investigated. Results revealed that incorporation of PHMB in chitosan/PEO solutions led to decrease in the zero-shear rate viscosity up to 20%. Moreover, increasing PHMB from 0.5mM to 1mM led to formation of thinner fibers with diameters ranging from 240nm to 60nm, respectively. Fourier transform infrared (FT-IR) spectrums indicated the functional groups of chitosan, PEO and PHMB in nanofibrous structure. Differential scanning calorimetry (DSC) thermograms indicated interaction of PHMB with PEO and chitosan through alteration in the thermal behavior of the nanofibers. Inhibition of the bacteria growth for both Escherichia coli and Staphylococcus aureus were achieved on the PHMB loaded nanofibers. Also, a burst release of PHMB from mats has been observed in the first hour. These findings suggest that there is a great potential in fabrication of biomaterials with incorporation of PHMB using electrospinning.