Cytotoxicity and antibacterial ability of scaffolds immobilized by polysaccharide/layered silicate composites

• Published in: Carbohydrate polymers 2013-02, Vol.92 (2), p.1880-1886
• Main Authors: Xin, Shangjing, Li, Xueyong, Ma, Zhaocheng, Lei, Zhanjun, Zhao, Jiemin, Pan, Siyi, Zhou, Xue, Deng, Hongbing
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - toxicity; Antibacterial; Applied sciences; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biocompatible Materials - toxicity; Biological and medical sciences; Carbohydrates; cellulose acetate; Chitosan; coatings; Cytotoxicity; Epidermis - cytology; Escherichia coli; Escherichia coli - drug effects; Exact sciences and technology; Fibers and threads; food packaging; Forms of application and semi-finished materials; Humans; Layer-by-layer; Medical sciences; nanofibers; Pectin; pectins; Polymer industry, paints, wood; Polysaccharides - chemistry; Polysaccharides - pharmacology; Polysaccharides - toxicity; scanning electron microscopes; Silicates - chemistry; Staphylococcus aureus; Staphylococcus aureus - drug effects; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology of polymers; Technology. Biomaterials. Equipments; X-ray diffraction; X-ray photoelectron spectroscopy
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.11.040

► Fabrication of pectin–OREC composites. ► Deposition of chitosan and pectin–OREC on cellulose acetate nanofibers via LBL. ► Characterization of LBL structured nanofibrous scaffolds. ► Study the bacterial inhibition abilities of the scaffolds. ► Investigate the cytotoxicity of scaffolds. Chitosan and pectin/organic rectorite (OREC) were initially deposited on the surface of cellulose acetate electrospun nanofibers by a layer-by-layer (LBL) technique to fabricate scaffolds for bacterial inhibition, and the cytotoxicity of the LBL structured scaffolds was also investigated. A couple of opposite charged material, pectin and OREC, were firstly used to fabricate the intercalated composites. The intercalated structure was determined by selected area electron diffraction. Field-emission scanning electron microscope, X-ray diffraction and X-ray photoelectron spectroscopy were applied for the characterization of LBL structured nanofibrous scaffolds. Antibacterial assay results showed that the diameters of the inhibition zone increased from 7.6 to 15.8mm for Escherichia coli, as well as from 7.4 to 14.2mm for Staphylococcus aureus. Finally, human epidermal (EP) cells grew well on the LBL films coating. These novel scaffolds could be an ideal candidate for wound dressings and food packaging.