Maize (Zea mays L.) constitutes a novel host to Sugarcane yellow leaf virus

Sugarcane yellow leaf virus (SCYLV) is a Polerovirus of the Luteoviridae family. In this study, SCYLV was transmitted by sugarcane aphids, Melanaphis sacchari, to two maize (Zea mays L.) lines. Infection of the maize plants was confirmed using RT-PCR with diagnostic primers YLS111 and YLS462, which...

Full description

Saved in:
Bibliographic Details
Published in:Canadian journal of plant pathology 2013, Vol.35 (1), p.68-74
Main Author: ElSayed, Abdelaleim Ismail
Format: Article
Language:English
Subjects:
Aphididae
corn
cultivars
genome
Genomes
genomics
gRNA
Infection
insect transmission
internodes
Luteoviridae
Melanaphis sacchari
Northern blotting
Plant viruses
Polymerase chain reaction
Primers
reverse transcriptase polymerase chain reaction
RNA
sugarcane
Sugarcane yellow leaf virus
transmission par les insectes
viral load
virus de la feuille jaune de la canne à sucre
yellow leaf
Zea mays
Zea mays L
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sugarcane yellow leaf virus (SCYLV) is a Polerovirus of the Luteoviridae family. In this study, SCYLV was transmitted by sugarcane aphids, Melanaphis sacchari, to two maize (Zea mays L.) lines. Infection of the maize plants was confirmed using RT-PCR with diagnostic primers YLS111 and YLS462, which produced the expected 351 bp band. The RT-PCR results were further confirmed by northern blot analysis, which revealed an accumulation of SCYLV at high levels in both maize lines. The northern blot analysis using RNA isolated from virus-infected maize plants also revealed that the genome of SCYLV is divided and contains genomic RNA (gRNA) and two subgenomic RNAs (sgRNAs) as observed in sugarcane, with estimated sizes of 6.0, 2.4 and 1.0 kb, respectively. The virus titre increased rapidly in the internodes of sugarcane cultivar H87-4094 from internode #1 (immature) to #7 (mature). This demonstrates the first successful transmission of Sugarcane yellow leaf virus by M. sacchari to maize plants.
ISSN:1715-2992
0706-0661
1715-2992
DOI:10.1080/07060661.2012.758177