Yamadazyma siamensis sp. nov., Yamadazyma phyllophila sp. nov. and Yamadazyma paraphyllophila sp. nov., three novel yeast species isolated from phylloplane in Thailand and Taiwan

• Published in: Antonie van Leeuwenhoek 2013-04, Vol.103 (4), p.777-788
• Main Authors: Kaewwichian, Rungluk, Yongmanitchai, Wichien, Kawasaki, Hiroko, Wang, Pi-Han, Yang, Shan-Hua, Limtong, Savitree
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Candida; Cluster Analysis; DNA, Fungal - chemistry; DNA, Fungal - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fabaceae - microbiology; Genes, rRNA; Life Sciences; Medical Microbiology; Microbiology; Molecular Sequence Data; Morphology; Mycological Typing Techniques; Original Paper; Phylogeny; Plant Leaves - microbiology; Plant Sciences; RNA, Fungal - genetics; RNA, Ribosomal - genetics; Saccharomycetales - classification; Saccharomycetales - genetics; Saccharomycetales - isolation & purification; Saccharomycetales - physiology; Saccharum - microbiology; Sequence Analysis, DNA; Soil Science & Conservation; Sugarcane; Taiwan; Taxonomy; Thailand; Yeast; Yeasts; Zea mays - microbiology
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-012-9860-6

Four strains representing three novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (DMKU-RK254 T ), corn leaves (DMKU-RK548 T ), bean leaves (K129) in Thailand and hengchun pencilwood leaves (TrB1-1 T ) in Taiwan. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region, the actin gene (ACT1) and the elongation factor 2 gene (EF2), the four strains were determined to represent novel Yamadazyma species although formation of ascospores was not observed. Strain DMKU-RK254 T was determined to be related to Candida diddensiae , Candida naeodendra and Candida kanchanaburiensis but with 1.8, 1.8 and 2.0 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, respectively. It was assigned to Yamadazyma siamensis sp. nov. (type strain DMKU-RK254 T  = BCC 50730 T  = NBRC 108901 T  = CBS 12573 T ). The sequences of the D1/D2 region of the LSU rRNA gene, the ITS region, ACT1 gene and EF2 gene of two strains (DMKU-RK548 T and K129) were identical but differed from that of strain TrB1-1 T by 0.6, 1.0, 3.3 and 5.9 % nucleotide substitutions, respectively. Therefore, the two strains (DMKU-RK548 T and K129) and strain TrB1-1 T were assigned to be two separate species. The closest species in terms of pairwise sequences similarity of the D1/D2 region to the two novel species was Yamadazyma philogaea but with 1.1–1.7 % nucleotide substitutions. The two strains (DMKU-RK548 T and K129) were assigned to Yamadazyma phyllophila sp. nov. (type strain DMKU-RK548 T  = BCC 50736 T  = NBRC 108906 T  = CBS 12572 T ) and the strain TrB1-1 T was named Yamadazyma paraphyllophila sp. nov. (type strain TrB1-1 T  = BCRC 23030 T  = CCTCC AY 204005 T  = CBS 9928 T ).