Sensing benzo[a]pyrene-DNA adducts formation via decrease of hybridization reaction

Toxicity of benzo[a]pyrene occurs because of the formation of covalent adducts with DNA guanines. In this work we report the attempt to detect this DNA-adduct using both an electrochemical assay based on gold nanoparticles and a surface plasmon resonance DNA sensor. Detection was achieved via inhibi...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2013-03, Vol.179, p.187-193
Main Authors: Lanzone, V., Scarano, S., Del Carlo, M., Corrado, F., Esposito, M., Minunni, M., Compagnone, D.
Format: Article
Language:English
Subjects:
Benzo[a]pyrene
DNA adducts
DNA sensors
Electrochemical DNA sensor
electrochemistry
hybridization
nanogold
oligonucleotides
PAHs
Surface plasmon resonance
toxicity
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Summary:Toxicity of benzo[a]pyrene occurs because of the formation of covalent adducts with DNA guanines. In this work we report the attempt to detect this DNA-adduct using both an electrochemical assay based on gold nanoparticles and a surface plasmon resonance DNA sensor. Detection was achieved via inhibition of the hybridization reaction of oligonucleotide sequences after formation of the covalent adduct. Detecting oligonucleotide sequences have been designed to get high reactivity vs formation of the adduct and benzo[a]-pyrene 7,8-dihydrodiol 9,10-epoxide has been used to generate adducts. Formation of adducts in the nanomolar range was evaluated using the surface plasmon resonance sensor with both the oligonucleotide sequences containing guanines.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2012.10.018