Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway

• Published in: Apoptosis (London) 2013, Vol.18 (1), p.28-42
• Main Authors: Wang, Gang, Han, Bing, Zhou, Haoxin, Wu, Linfeng, Wang, Yongwei, Jia, Guang, Lv, Jiachen, Cheng, Zhuoxin, Pan, Shangha, Liu, Ji, Zhou, Yinan, Sun, Bei
• Format: Article
• Language: English
• Subjects: Acinar cells; Aldehydes - blood; Alkynes - pharmacology; Animals; Apoptosis; Apoptosis - drug effects; Bax protein; Bcl-2 protein; Biochemistry; Biomedical and Life Sciences; Biomedicine; Blood; Cancer Research; Caspase-3; Caspases - metabolism; Cell Biology; Cystathionine gamma-Lyase - antagonists & inhibitors; Cytochrome; Cytochrome c; Enzyme Inhibitors - pharmacology; Extracellular signal-regulated kinase; Gene expression; Glycine - analogs & derivatives; Glycine - pharmacology; Hydrogen sulfide; Hydrogen Sulfide - blood; Hydrogen Sulfide - metabolism; Inflammation; Interleukin 10; Interleukin-10 - blood; Male; mRNA; NF- Kappa B protein; Oncology; Original Paper; Pancreas; Pancreas - cytology; Pancreas - pathology; Pancreatitis; Pancreatitis, Acute Necrotizing - pathology; Pancreatitis, Acute Necrotizing - prevention & control; Peroxidase; Peroxidase - metabolism; Phosphorylation; Rats; Rats, Wistar; SAP protein; Sodium; Sulfides - pharmacology; Tumor necrosis factor- alpha; Tumor Necrosis Factor-alpha - blood; Virology
• ISSN: 1360-8185; 1573-675X
• DOI: 10.1007/s10495-012-0770-x

We aimed to investigate the relationship between the synthesis of hydrogen sulfide (H 2 S) and the pancreatic acinar cell apoptosis in severe acute pancreatitis (SAP) rats, as well as analyse the potential apoptotic pathway involved in this process. Sixty rats had been equally divided into four groups: sham, SAP, SAP + sodium hydrosulfide (NaHS) and SAP + DL-propargylglycine (PAG). 24 h after SAP induction, all surviving animals of each group were sacrificed to collect blood and tissue samples for the following measurements: the level of serum H 2 S as well as the levels of tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), H 2 S synthesizing activity, CSE mRNA and protein expression, maleic dialdehyde (MDA) and myeloperoxidase (MPO) activity, the expression of Bax, Bcl-2, caspase-3, -8 and -9, the release of cytochrome c and the activation of nuclear factor-kappa B (NF-κB), ERK1/2, JNK1/2 and p38 in pancreas. Furthermore, in situ detection of cell apoptosis was examined and the severity of pancreatic damage was analyzed by pathological grading and scoring. Results Significant differences in every index except IL-10 had been found between the SAP, NaHS and PAG groups ( P