Association with Leptin Gene c.-2548 G>A Polymorphism, Serum Leptin Levels, and Body Mass Index in Turkish Obese Patients

Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene ( LEP ), which encodes for leptin, have been described. However, its association with obesity is still c...

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Bibliographic Details
Published in:Cell biochemistry and biophysics 2013-03, Vol.65 (2), p.243-247
Main Authors: Sahin, Deniz Say, Tumer, Cemil, Demir, Cemil, Celik, M. Murat, Celik, Mustafa, Ucar, Edip, Gunesacar, Ramazan
Format: Article
Language:English
Subjects:
Adult
Alleles
Biochemistry
Biological and Medical Physics
Biomarkers - blood
Biomedical and Life Sciences
Biophysics
Biotechnology
Body Mass Index
Cell Biology
Cholesterol - blood
Colorimetry
Energy expenditure
Enzyme-Linked Immunosorbent Assay
Food intake
Gene Frequency
Gene polymorphism
Genotype
Genotypes
Hormones
Humans
Leptin
Leptin - blood
Leptin - genetics
Life Sciences
Lipids
Lipoproteins, LDL - blood
Middle Aged
Obesity
Obesity - blood
Obesity - genetics
Original Paper
Pharmacology/Toxicology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Triglycerides - blood
Turkey
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Summary:Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene ( LEP ), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefore, in the present study, we aimed to investigate whether LEP c.-2548 G>A polymorphism was associated with serum leptin levels, lipid parameters, and body mass index in Turkish obese patients. Forty-seven obese patients and 48 healthy individuals were included in the study. Blood samples were collected for DNA extraction. LEP c.-2548 G>A polymorphism were detected using polymerase chain reaction–restriction fragment length polymorphism technique. Serum leptin levels and lipid parameters were measured by ELISA and enzyme colorimetric assay techniques, respectively. GA or AA genotypes and A allele carrier frequencies of the c.-2548 G>A polymorphism in the LEP were higher in obese (38.3, 34.0 and 72.3 %) when compared with controls (14.6, 12.5, and 27.1 %; p  = 0.011, 0.016, and 0.002, respectively). On the other hand, AA or AG genotypes were also related to increased serum leptin levels ( p  
ISSN:1085-9195
1559-0283
DOI:10.1007/s12013-012-9427-1