Characterization of a refinement of the apylorus ligationa model of rat gastric ulceration resulting in ano paina and a more specific pharmacological response

• Published in: Journal of pharmacological and toxicological methods 2013-04, Vol.67 (2), p.121-128
• Main Authors: Monteiro, Karin M, Spindola, Humberto M, Possenti, Ana, Tinti, Sirlene V, Ruiz, Ana LTG, Longato, Giovanna B, Fiorito, Giovanna F, Marchetti, Gabriela M, Shiozawa, Larissa, Piloni, Barbara U, de Oliveira, Ana C, Miyagawa, Livia M, Carvalho, Joao E
• Format: Article
• Language: English
• ISSN: 1056-8719

Introduction: The pharmacological assessment of the factors for gastric protection of a test substance should involve experimental models that can determine the involvement of cytoprotective factors, as well as their influence on the secretion of hydrochloric acid. The original protocol of pylorus ligation in rats proposed by Shay et al. in 1945, still in use today, provides a latency time of 240 min without considering the effect of postoperative pain in the mechanisms of peptic ulcer. This paper proposes a modification of this experimental protocol by eliminating the pain throughout the postoperative period, as a refinement of the test with consequent improvement of the pharmacological response. Methods: Adult male Wistar/Uni rats underwent surgical ligation of the pylorus and were kept anesthetized throughout the experimental period (4 h) in contrast to the other experimental groups that followed the original protocol proposed by Shay et al., 1945. Results: We were able to determine effective doses for a positive control, as well as of a variety of secretagogues in the new experimental protocol proposed.