Mass spectral measurements of the apoHDL in horse (Equus caballus) cerebrospinal fluid

As a continuation of our proteogenomic studies of equine apolipoproteins, we have obtained molecular masses for several of the apolipoproteins associated with the HDL in horse cerebrospinal fluid (CSF). Using electrospray-ionization mass spectrometry (ESI-MS), we report on values for apolipoproteins...

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Bibliographic Details
Published in:Comparative biochemistry and physiology. Part D, Genomics & proteomics Genomics & proteomics, 2012-06, Vol.7 (2), p.172-174
Main Authors: Puppione, Donald L., Della Donna, Lorenza, Bassilian, Sara, Souda, Puneet, MacDonald, Melinda H., Whitelegge, Julian P.
Format: Article
Language:English
Subjects:
Acylated apoA-I
Animals
apoE
Apolipoprotein A-I - cerebrospinal fluid
Apolipoprotein A-I - chemistry
Apolipoprotein A-II - cerebrospinal fluid
Apolipoprotein A-II - chemistry
Dimeric apoA-II
Equus caballus
Horses - cerebrospinal fluid
Male
Mass spectroscopy
Molecular Weight
Spectrometry, Mass, Electrospray Ionization
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Summary:As a continuation of our proteogenomic studies of equine apolipoproteins, we have obtained molecular masses for several of the apolipoproteins associated with the HDL in horse cerebrospinal fluid (CSF). Using electrospray-ionization mass spectrometry (ESI-MS), we report on values for apolipoproteins, A-I and A-II, as well as acylated apoA-I. In comparison with our previously published data on equine plasma apolipoproteins, there appears to be a higher percentage of acylated apoA-I in the CSF than in plasma. As was the case in plasma, apoA-II circulates as a homodimer. These studies also revealed a protein with a mass of 34,468Da that we are speculating is the value for horse apoE.
ISSN:1744-117X
1878-0407
DOI:10.1016/j.cbd.2012.02.002