Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0

► Light-induced silencing of neural activity in genetically defined cell populations. ► We generated new mouse strains that express eNpHR2-EYFP fusion proteins. ► In these mice, Cre/Flp recombination induced a high-level of eNpHR2-EYFP expression. Temporally precise inhibition of genetically defined...

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Bibliographic Details
Published in:Neuroscience research 2013-01, Vol.75 (1), p.53-58
Main Authors: Imayoshi, Itaru, Tabuchi, Sawako, Hirano, Kyoko, Sakamoto, Masayuki, Kitano, Satsuki, Miyachi, Hitoshi, Yamanaka, Akihiro, Kageyama, Ryoichiro
Format: Article
Language:English
Subjects:
Action Potentials - physiology
Animals
Cre
eNpHR
Female
Flp
Gene Knock-In Techniques
Halorhodopsins - biosynthesis
Halorhodopsins - genetics
Immunohistochemistry
Light
Male
Mice
Mice, Transgenic
Neurons - metabolism
Optogenetics
Optogenetics - methods
Patch-Clamp Techniques
Rosa26
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Summary:► Light-induced silencing of neural activity in genetically defined cell populations. ► We generated new mouse strains that express eNpHR2-EYFP fusion proteins. ► In these mice, Cre/Flp recombination induced a high-level of eNpHR2-EYFP expression. Temporally precise inhibition of genetically defined cell populations in intact nervous systems has been enabled by the microbial halorhodopsin NpHR, a fast, light-activated chloride pump. Here, we report the generation of new mouse strains that express eNpHR2-EYFP fusion proteins after Cre- and/or Flp-mediated recombination to silence neural activity in vivo. In these mouse strains, Cre/Flp recombination induced a high-level of eNpHR2-EYFP expression. Slice whole-cell patch clamp experiments confirmed that eNpHR2-EYFP-expressing neurons could be optically hyperpolarized and inhibited from firing action potentials. Thus, these mouse strains offer powerful tools for light-induced silencing of neural activity in genetically defined cell populations.
ISSN:0168-0102
1872-8111
DOI:10.1016/j.neures.2012.03.008