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A Network Model of the Molecular Organization of Chromatin in Drosophila
Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associ...
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Published in: | Molecular cell 2013-02, Vol.49 (4), p.759-771 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associated with chromatin, which all display specific genomic binding patterns. Bayesian network modeling of the binding profiles of these and 70 known chromatin components yields a detailed blueprint of the in vivo chromatin protein network. We demonstrate functional compartmentalization of this network, and predict functions for most of the previously unknown chromatin proteins, including roles in DNA replication and repair, and gene activation and repression.
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► DamID identifies 42 previously unknown chromatin proteins ► Binding maps of new and known chromatin components yield a chromatin network model ► The network model predicts functions of most of the 42 new chromatin proteins ► The chromatin protein network is functionally compartmentalized |
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ISSN: | 1097-2765 1097-4164 |
DOI: | 10.1016/j.molcel.2013.01.040 |