Genetic control and mapping of Solanum chilense LA1932, LA1960 and LA1971-derived resistance to Tomato yellow leaf curl disease

• Published in: Euphytica 2013-04, Vol.190 (2), p.203-214
• Main Authors: DE CASTRO, Ana Pérez, JULIAN, Olga, DIEZ, María José
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Analysis; Begomovirus; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Breeding; chromosome 6; Disease resistance; Ethylenediaminetetraacetic acid; Fundamental and applied biological sciences. Psychology; Gene loci; Gene mapping; Genetic control; Genetic research; Genetics and breeding of economic plants; Health aspects; Infection; Inoculum; Life Sciences; Lycopersicon esculentum; Plant diseases; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant resistance; Plant Sciences; Pressure; Recombinants; Solanum; Tomatoes; Varietal selection. Specialized plant breeding, plant breeding aims; Virus diseases; yellow leaf
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-012-0792-z

Tomato yellow leaf curl disease (TYLCD) is caused by a complex of begomovirus. Breeding for resistance to this disease has mainly been based on Ty - 1 gene, derived from Solanum chilense LA1969. Commercial varieties available to date still develop symptoms and suffer yield losses with high inoculum pressure and early infections. It is of interest to incorporate in breeding programs resistance from the different available sources. Lines with resistance to TYLCD derived from S . chilense accessions LA1932, LA1960 and LA1971 were previously developed. The objectives of this work were to study the genetic control of the resistance derived from these accessions and to map the resistance loci. Response to viral infection was assayed in segregating generations derived from these sources. Results obtained were compatible with a monogenic control of resistance. A total of 94 markers were used to locate the S . chilense introgressions in each of the lines. Only the presence of a large introgression in chromosome 6 was common to all lines. Analysis of recombinants allowed localizing the resistance loci in an interval of approximately 25 cM, also common to all five families. This interval includes the region to which two previously S . chilense -derived TYLCD resistance loci have been mapped, the Ty - 1 / Ty - 3 region. This is the first report of LA1960 and LA1971-derived TYLCV resistance loci to be located on chromosome 6. Further work will be done to fine map the loci found in the present work, in order to determine if they are indeed located in the Ty - 1 / Ty - 3 region.