Kinetics analysis of the enzymatic hydrolysis of cellulose from straw stalk

The effects of several parameters on the simultaneous saccharification and fermentation of straw stalk for ethanol fuel production were investigated on the basis of orthogonal experiments. The parameters include temperature and time of fermentation, quantity and proportion of yeast inoculation, as w...

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Bibliographic Details
Published in:Acta geochimica 2013-03, Vol.32 (1), p.41-46
Main Authors: Chen, Kun, Xu, Longjun, Bi, Zhiming, Fu, Zhilei
Format: Article
Language:English
Subjects:
Biomass
Cellulase
Cellulose
Earth and Environmental Science
Earth Sciences
Ethanol
Fermentation
Geochemistry
Hydrolysis
Kinetics
Yeasts
乙醇燃料
动力学分析
反应速率常数
发酵时间
发酵生产
秸秆
纤维素酶
酶水解
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Summary:The effects of several parameters on the simultaneous saccharification and fermentation of straw stalk for ethanol fuel production were investigated on the basis of orthogonal experiments. The parameters include temperature and time of fermentation, quantity and proportion of yeast inoculation, as well as cellulase dosage. An ethanol yield of 0.183 g/g was obtained from the straw stalk pretreated with diluted acid under determined optimum conditions. These conditions were： fermentation temperature： 38℃; fermentation time： 72 h; yeast inoculation quantity： 15%; yeast inoculation proportion： 2：1; and cellulose enzyme dosage： 20 U/g. The relationship between ethanol concentration c and fermentation time t is presented as follows, c=abt/（l＋bt）. The rate constant k of straw stalk hy- drolysis by the cellulose enzyme depends on hydrolysis time, as described by k=kfh. Therefore, straw stalk hy- drolysis reaction by the cellulose enzyme is fractal-like.
ISSN:1000-9426
2096-0956
1993-0364
2365-7499
DOI:10.1007/s11631-013-0605-7