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Kinetics analysis of the enzymatic hydrolysis of cellulose from straw stalk
The effects of several parameters on the simultaneous saccharification and fermentation of straw stalk for ethanol fuel production were investigated on the basis of orthogonal experiments. The parameters include temperature and time of fermentation, quantity and proportion of yeast inoculation, as w...
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Published in: | Acta geochimica 2013-03, Vol.32 (1), p.41-46 |
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creator | Chen, Kun Xu, Longjun Bi, Zhiming Fu, Zhilei |
description | The effects of several parameters on the simultaneous saccharification and fermentation of straw stalk for ethanol fuel production were investigated on the basis of orthogonal experiments. The parameters include temperature and time of fermentation, quantity and proportion of yeast inoculation, as well as cellulase dosage. An ethanol yield of 0.183 g/g was obtained from the straw stalk pretreated with diluted acid under determined optimum conditions. These conditions were: fermentation temperature: 38℃; fermentation time: 72 h; yeast inoculation quantity: 15%; yeast inoculation proportion: 2:1; and cellulose enzyme dosage: 20 U/g. The relationship between ethanol concentration c and fermentation time t is presented as follows, c=abt/(l+bt). The rate constant k of straw stalk hy- drolysis by the cellulose enzyme depends on hydrolysis time, as described by k=kfh. Therefore, straw stalk hy- drolysis reaction by the cellulose enzyme is fractal-like. |
doi_str_mv | 10.1007/s11631-013-0605-7 |
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The parameters include temperature and time of fermentation, quantity and proportion of yeast inoculation, as well as cellulase dosage. An ethanol yield of 0.183 g/g was obtained from the straw stalk pretreated with diluted acid under determined optimum conditions. These conditions were: fermentation temperature: 38℃; fermentation time: 72 h; yeast inoculation quantity: 15%; yeast inoculation proportion: 2:1; and cellulose enzyme dosage: 20 U/g. The relationship between ethanol concentration c and fermentation time t is presented as follows, c=abt/(l+bt). The rate constant k of straw stalk hy- drolysis by the cellulose enzyme depends on hydrolysis time, as described by k=kfh. Therefore, straw stalk hy- drolysis reaction by the cellulose enzyme is fractal-like.</description><identifier>ISSN: 1000-9426</identifier><identifier>ISSN: 2096-0956</identifier><identifier>EISSN: 1993-0364</identifier><identifier>EISSN: 2365-7499</identifier><identifier>DOI: 10.1007/s11631-013-0605-7</identifier><language>eng</language><publisher>Heidelberg: SP Science Press</publisher><subject>Biomass ; Cellulase ; Cellulose ; Earth and Environmental Science ; Earth Sciences ; Ethanol ; Fermentation ; Geochemistry ; Hydrolysis ; Kinetics ; Yeasts ; 乙醇燃料 ; 动力学分析 ; 反应速率常数 ; 发酵时间 ; 发酵生产 ; 秸秆 ; 纤维素酶 ; 酶水解</subject><ispartof>Acta geochimica, 2013-03, Vol.32 (1), p.41-46</ispartof><rights>Science Press, Institute of Geochemistry, CAS and Springer-Verlag Berlin Heidelberg 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a398t-33a3f261b9897b268a2bfe7bb4be81b10a51361d5965a010adcca67afd56ee7c3</citedby><cites>FETCH-LOGICAL-a398t-33a3f261b9897b268a2bfe7bb4be81b10a51361d5965a010adcca67afd56ee7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85079X/85079X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Chen, Kun</creatorcontrib><creatorcontrib>Xu, Longjun</creatorcontrib><creatorcontrib>Bi, Zhiming</creatorcontrib><creatorcontrib>Fu, Zhilei</creatorcontrib><title>Kinetics analysis of the enzymatic hydrolysis of cellulose from straw stalk</title><title>Acta geochimica</title><addtitle>Chin. J. Geochem</addtitle><addtitle>Chinese Journal of Geochemistry</addtitle><description>The effects of several parameters on the simultaneous saccharification and fermentation of straw stalk for ethanol fuel production were investigated on the basis of orthogonal experiments. The parameters include temperature and time of fermentation, quantity and proportion of yeast inoculation, as well as cellulase dosage. An ethanol yield of 0.183 g/g was obtained from the straw stalk pretreated with diluted acid under determined optimum conditions. These conditions were: fermentation temperature: 38℃; fermentation time: 72 h; yeast inoculation quantity: 15%; yeast inoculation proportion: 2:1; and cellulose enzyme dosage: 20 U/g. The relationship between ethanol concentration c and fermentation time t is presented as follows, c=abt/(l+bt). The rate constant k of straw stalk hy- drolysis by the cellulose enzyme depends on hydrolysis time, as described by k=kfh. 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subjects | Biomass Cellulase Cellulose Earth and Environmental Science Earth Sciences Ethanol Fermentation Geochemistry Hydrolysis Kinetics Yeasts 乙醇燃料 动力学分析 反应速率常数 发酵时间 发酵生产 秸秆 纤维素酶 酶水解 |
title | Kinetics analysis of the enzymatic hydrolysis of cellulose from straw stalk |
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