Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-Coenzyme A: Diacylglycerol acyltransferase family in the diatom Phaeodactylum tricornutum

► We identified four type 2 DGAT2 proteins from Phaeodactylum tricornutum. ► TAG-deficient phenotype of yeast mutant was complemented by PtDGAT2B. ► Expression patterns of four PtDGAT2 genes were examined. ► PtDGAT2B expression can increase the proportion of unsaturated fatty acids in yeast TAG. Dia...

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Bibliographic Details
Published in:FEBS letters 2013-03, Vol.587 (5), p.481-487
Main Authors: Gong, Yangmin, Zhang, Junping, Guo, Xiaojing, Wan, Xia, Liang, Zhuo, Hu, Chuan Jiong, Jiang, Mulan
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Bacillariophyceae
Conserved Sequence
DGAT2
Diacylglycerol O-Acyltransferase - biosynthesis
Diacylglycerol O-Acyltransferase - chemistry
Diacylglycerol O-Acyltransferase - genetics
Diatoms - enzymology
Diatoms - metabolism
Fatty acid
Gene Expression
Molecular Sequence Data
Phaeodactylum tricornutum
Phylogeny
Pichia
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Substrate Specificity
Triacylglycerol
Triglycerides - biosynthesis
Triglycerides - metabolism
Yeast
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Summary:► We identified four type 2 DGAT2 proteins from Phaeodactylum tricornutum. ► TAG-deficient phenotype of yeast mutant was complemented by PtDGAT2B. ► Expression patterns of four PtDGAT2 genes were examined. ► PtDGAT2B expression can increase the proportion of unsaturated fatty acids in yeast TAG. Diacylglycerol acyltransferase (DGAT) plays a pivotal role in triacylglycerol (TAG) formation in some oleaginous organisms. We describe here the identification of a type 2 DGAT (PtDGAT2B) in the diatom Phaeodactylum tricornutum that contains four putative type 2 acyl-CoA:DGATs, sharing little sequence similarity with each other. TAG synthesis and lipid body formation could be completely restored in a Saccharomyces cerevisiae TAG-deficient quadruple mutant by expressing PtDGAT2B. Up-regulation of PtDGAT2B precedes the accumulation of TAG. Functional analysis of enzyme activity in vivo demonstrated that expression of PtDGAT2B can increase the proportion of unsaturated C16 and C18 fatty acids in yeast TAG.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2013.01.015