Digging into the extracellular matrix of a complex microbial community using a combined metagenomic and metaproteomic approach

Knowledge about identity and function of extracellular polymeric substances (EPS) in complex microbial communities is sparse, although these components have a large influence on the function of the microbial communities. We investigated the presence of selected genes potentially involved in EPS prod...

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Bibliographic Details
Published in:Water science and technology 2013-01, Vol.67 (7), p.1650-1656
Main Authors: ALBERTSEN, M, STENSBALLE, A, NIELSEN, K. L, NIELSEN, P. H
Format: Article
Language:English
Subjects:
Alginates
Alginic acid
Amyloid - metabolism
Applied sciences
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Communities
Exact sciences and technology
Extracellular
Extracellular matrix
Extracellular Matrix - metabolism
General purification processes
Genes
Glucuronic Acid - biosynthesis
Hexuronic Acids
Identification
Mass spectrometry
Mass spectroscopy
Metagenome
Microbial activity
Microbial Consortia - genetics
Microorganisms
Phosphorus
Phosphorus removal
Pollution
Polysaccharides, Bacterial - biosynthesis
Proteins
Removal
Seaweed meal
Wastewater
Wastewater treatment
Wastewater treatment plants
Wastewaters
Water treatment and pollution
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Summary:Knowledge about identity and function of extracellular polymeric substances (EPS) in complex microbial communities is sparse, although these components have a large influence on the function of the microbial communities. We investigated the presence of selected genes potentially involved in EPS production in a 145 Mbp metagenome prepared by Illumina sequencing from a full-scale wastewater treatment plant carrying out enhanced biological phosphorus removal (EBPR). A range of genes involved in alginate production was identified and assigned mainly to bacteria from the phylum Bacteroidetes. Furthermore, several proteins in the EPS matrix were extracted, purified and identified by mass spectrometry. By using the metagenome as a reference for the metaproteomic analysis, more proteins were identified compared to using only publicly available databases. This illustrates the low degree of similarity between the bacteria in the EBPR community and the sequenced bacteria in the public databases. Hence, the combination of metagenomics and metaproteomics presented here is needed to investigate the identity of the proteins in the EPS matrix.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2013.030