Structural evaluation of sugar cane bagasse steam pretreated in the presence of CO sub(2) and SO sub(2)

• Published in: Biotechnology for biofuels 2012-01, Vol.5 (1), p.36-36
• Main Authors: Corrales, Roberta Cristina Novaes Reis, Mendes, Fabiana Magalhaes Teixeira, Perrone, Clarissa Cruz, Sant'Anna, Celso, de Souza, Wanderley, Abud, Yuri, Bon, Elba Pinto da Silva, Ferreira-Leitao, Viridiana
• Format: Article
• Language: English
• ISSN: 1754-6834; 1754-6834
• DOI: 10.1186/1754-6834-5-36

Background: Previous studies on the use of SO sub(2) and CO sub(2) as impregnating agent for sugar cane bagasse steam treatment showed comparative and promising results concerning the cellulose enzymatic hydrolysis and the low formation of the inhibitors furfural and hydroxymethylfurfural for the use of CO sub(2) at 205 degree C/15 min or SO sub(2) at 190 degree C/5 min. In the present study sugar cane bagasse materials pretreated as aforementioned were analyzed by scanning and transmission electron microscopy (SEM and TEM), X-Ray Diffraction (XRD) and Infrared (FTIR spectroscopy) aiming a better understanding of the structural and chemical changes undergone by the pretreated materials. Results: SEM and TEM data showed that the structural modifications undergone by the pretreatment with CO sub(2) were less pronounced in comparison to that using SO sub(2,) which can be directly related to the combined severity of each pretreatment. According to XRD data, untreated bagasse showed, as expected, a lower crystallinity index (CI = 48.0%) when compared to pretreated samples with SO sub(2) (CI = 65.5%) or CO sub(2) (CI = 56.4%), due to the hemicellulose removal of 68.3% and 40.5%, respectively. FTIR spectroscopy supported SEM, TEM and XRD results, revealing a more extensive action of SO sub(2). Conclusions: The SEM, TEM, XRD and FTIR spectroscopy techniques used in this work contributed to structural and chemical analysis of the untreated and pretreated bagasse. The images from SEM and TEM can be related to the severity of SO sub(2) pretreatment, which is almost twice higher. The crystallinity index values obtained from XRD showed that pretreated materials have higher values when compared with untreated material, due to the partial removal of hemicellulose after pretreatment. FTIR spectroscopy supported SEM, TEM and XRD results. CO sub(2) can actually be used as impregnating agent for steam pretreatment, although the present study confirmed a more extensive action of SO sub(2).