Development and haematotoxicological evaluation of doped hydroxyapatite based multimodal nanocontrast agent for near-infrared, magnetic resonance and X-ray contrast imaging

Abstract Multimodal molecular imaging provides both anatomical and molecular information, aiding early stage detection and better treatment planning of diseased conditions. Here, we report development and nanotoxicity evaluation of a novel hydroxyapatite nanoparticle (nHAp) based multimodal contrast...

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Bibliographic Details
Published in:Nanotoxicology 2012-09, Vol.6 (6), p.652-666
Main Authors: Ashokan, Anusha, Chandran, Parwathy, Sadanandan, Aparna R., Koduri, Chaitanya K., Retnakumari, Archana P., Menon, Deepthy, Nair, Shantikumar, Koyakutty, Manzoor
Format: Article
Language:English
Subjects:
Adult Stem Cells
Blood Coagulation - drug effects
Cell Proliferation - drug effects
Cell Survival - drug effects
Contrast Media - chemistry
Contrast Media - pharmacology
Cytokines - metabolism
Durapatite - chemistry
Durapatite - pharmacology
Flow Cytometry
Gadolinium - chemistry
haematotoxicity
Hemolysis - drug effects
Humans
Hydrogen-Ion Concentration
Hydroxyapatite nanoparticles
Inflammation
Leukocytes, Mononuclear
Magnetic Resonance Imaging
Mesenchymal Stromal Cells
Molecular Imaging - methods
MRI
Nanoparticles - chemistry
near-infrared imaging
Platelet Activation - drug effects
Platelet Aggregation - drug effects
Radiography
Spectroscopy, Near-Infrared
X-ray contrast
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Summary:Abstract Multimodal molecular imaging provides both anatomical and molecular information, aiding early stage detection and better treatment planning of diseased conditions. Here, we report development and nanotoxicity evaluation of a novel hydroxyapatite nanoparticle (nHAp) based multimodal contrast agent for combined near-infrared (NIR), MR and X-ray imaging. Under optimised wet-chemical conditions, we achieved simultaneous doping of nHAp (size ∼50 nm) with indocyanine green and Gd3+ contributing to NIR contrast (∼750-850 nm), paramagnetic behaviour and X-ray absorption suitable for NIR, MR and X-ray contrast imaging, respectively. Haematocompatibility studies using stem cell viability, haemolysis, platelet activation, platelet aggregation and coagulation time analysis indicated excellent compatibility of doped nHAp (D-nHAp). Further, the immunogenic function studies using human lymphocytes (in vitro) showed that D-nHAp caused no adverse effects. Collectively, our studies suggest that D-nHAp with excellent biocompatibility and multifunctional properties is a promising nanocontrast agent for combined NIR, MR and X-ray imaging applications.
ISSN:1743-5390
1743-5404
DOI:10.3109/17435390.2011.600839