Lupenone Isolated from Adenophora triphylla var. japonica Extract Inhibits Adipogenic Differentiation through the Downregulation of PPARγ in 3T3-L1 Cells

Adenophora triphylla var. japonica (Campanulaceae) is known to have anti‐inflammatory and anti‐tussive effects. Dysfunction of adipocytes and adipose tissue in obesity is related to various inflammatory cytokines or adipokines. In this study, we investigated whether lupenone isolated from A. triphyl...

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Bibliographic Details
Published in:Phytotherapy research 2013-05, Vol.27 (5), p.761-766
Main Authors: Ahn, Eun-Kyung, Oh, Joa Sub
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Adenophora triphylla var. japonica
Adipocytes - cytology
Adipocytes - drug effects
adipogenesis
Adipogenesis - drug effects
adipogenic marker genes
Animals
Campanulaceae - chemistry
CCAAT-Enhancer-Binding Protein-alpha - metabolism
Cell Differentiation - drug effects
Chromans - pharmacology
Down-Regulation
HEK293 Cells
Humans
lupenone
Mice
peroxisome proliferator-activated receptor γ (PPAR γ)
Plant Extracts - pharmacology
PPAR gamma - metabolism
Thiazolidinediones - pharmacology
Transcriptional Activation - drug effects
Triterpenes - isolation & purification
Triterpenes - pharmacology
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Summary:Adenophora triphylla var. japonica (Campanulaceae) is known to have anti‐inflammatory and anti‐tussive effects. Dysfunction of adipocytes and adipose tissue in obesity is related to various inflammatory cytokines or adipokines. In this study, we investigated whether lupenone isolated from A. triphylla var. japonica extract inhibits adipocyte differentiation and expression of adipogenic marker genes in 3T3‐L1 preadipocytes. We demonstrated that lupenone resulted in a significant reduction in lipid accumulation and expression of adipogenic marker genes in a dose‐dependent manner. In addition, lupenone decreased the transcriptional activity of peroxisome proliferator‐activated receptor γ (PPARγ) induced by troglitazone, and we also demonstrated that lupenone suppressed the PPARγ and CCAAT‐enhancer‐binding protein α (C/EBPα) protein levels. These findings demonstrated that lupenone isolated from A. triphylla var. japonica extract effectively inhibited adipocyte differentiation through downregulation of related transcription factor, particularly the PPARγ gene. Copyright © 2012 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.4779