A retrospective review of UroVysion fish interpretations over 8.6 years: A major shift in the patient test population

UroVysion FISH detects chromosomal aberrations associated with urothelial carcinoma. In our laboratory, UroVysion FISH was initially evaluated manually with a change to image‐aided interpretation using the BioView Duet imaging system. This retrospective study examined diagnostic findings over an 8.6...

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Bibliographic Details
Published in:Diagnostic cytopathology 2013-05, Vol.41 (5), p.437-447
Main Authors: Denice Smith, G., Sangle, Nikhil A., Wilson, Andrew, Chadwick, Barbara E., Bentz, Joel S., Gopez, Evelyn V., Pyne, Michael T., Tripp, Sheryl R., Layton, Michelle, Pasi, Alexandra, Jarboe, Elke A., Collins, Brian T.
Format: Article
Language:English
Subjects:
Adult
BioView duet imaging system
Carcinoma - diagnosis
Carcinoma - genetics
Chromosome Aberrations
Early Detection of Cancer
False Positive Reactions
Female
Humans
image-aided interpretation
In Situ Hybridization, Fluorescence
Male
manual interpretation
Middle Aged
patient population
retrospective review
Retrospective Studies
Sex Factors
Urologic Neoplasms - diagnosis
Urologic Neoplasms - genetics
UroVysion FISH
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Summary:UroVysion FISH detects chromosomal aberrations associated with urothelial carcinoma. In our laboratory, UroVysion FISH was initially evaluated manually with a change to image‐aided interpretation using the BioView Duet imaging system. This retrospective study examined diagnostic findings over an 8.6 year period, with 1,869 manual interpretations over 4.8 years and 3,936 image‐aided interpretations over 3.8 years. Although the initial goal was to evaluate possible impacts of the imaging system on diagnostic interpretations, the most important finding was that the demographics of the test population changed significantly. Female specimens increased incrementally from an average of 29% compared to 43% of the samples during periods of manual interpretation versus image‐aided interpretation, respectively. The shift may reflect a gradual increase in the percentage of low‐risk hematuria patients being evaluated for initial diagnosis of bladder cancer, rather than bladder cancer recurrence. Interpretation rates, evaluated separately for males and females, changed significantly over the test period. Male interpretation results were negative (75.1 vs. 67%), positive (18.6 vs. 14.6%), unsatisfactory (5.0 vs. 16.9%), and equivocal (1.4 vs. 1.5%) during periods of manual versus image‐aided interpretation, respectively (Fisher Exact Test P‐value =
ISSN:8755-1039
1097-0339
DOI:10.1002/dc.22881